X0306 4 2022-06-25 0 0001492674 T2 Biosystems, Inc. TTOO 0001292650 Elsbree David B 101 HARTWELL AVENUE LEXINGTON MA 02421 1 0 0 0 Common Stock 2022-06-25 4 M 0 73224 A 276205 D Common Stock 10000 I By spouse Restricted Stock Units 2022-06-25 4 M 0 73224 0.00 D Common Stock 73224 0 D Each restricted stock unit represents a contingent right to receive one share of the Issuer's common stock. The reporting person disclaims ownership of these securities and this report shall not be deemed an admission that the reporting person is the beneficial owner of such securities for purposes of Section 16 or for any other purpose. On June 25, 2021, the reporting person was granted 73,224 restricted stock units that vested on the earlier of (i) June 25, 2022, and (ii) the date of the next annual meeting of stockholders. /s/ John Sprague, Attorney-in-Fact 2022-06-28