X0306 4 2021-07-01 0 0001492674 T2 Biosystems, Inc. TTOO 0001181189 CUMMING JOHN W 101 HARTWELL AVE LEXINGTON MA 02421 1 0 0 0 Common Stock 2021-07-01 4 S 0 18182 1.16 D 50852 D The transaction was effected pursuant to a Rule 10b5-1 trading plan adopted by the reporting person on May 20, 2021, to cover payment of tax liability incident to the vesting of restricted stock units. The price reported is a weighted average price. These shares were sold in multiple transactions at per share prices ranging from $1.16 to $1.19. The reporting person undertakes to provide upon request to the SEC staff, the Issuer, or any stockholder of the Issuer, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. /s/ John Sprague, Attorney-in-Fact 2021-07-02