<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2016-12-30</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001476963</issuerCik>
        <issuerName>HEAT BIOLOGICS, INC.</issuerName>
        <issuerTradingSymbol>HTBX</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001381450</rptOwnerCik>
            <rptOwnerName>Wolf Jeffrey Alan</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O 801 CAPITOLA DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>DURHAM</rptOwnerCity>
            <rptOwnerState>NC</rptOwnerState>
            <rptOwnerZipCode>27713</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Chief Executive Officer</officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

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                <value>Common Stock</value>
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            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
                <transactionShares>
                    <value>75000</value>
                    <footnoteId id="F1"/>
                </transactionShares>
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                </transactionAcquiredDisposedCode>
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                    <value>79881</value>
                </sharesOwnedFollowingTransaction>
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                <directOrIndirectOwnership>
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                <value>Common Stock</value>
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                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
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                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnotes</value>
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                    <footnoteId id="F5"/>
                </natureOfOwnership>
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            <securityTitle>
                <value>Common Stock</value>
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                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnotes</value>
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                    <footnoteId id="F5"/>
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            </ownershipNature>
        </nonDerivativeHolding>
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    <derivativeTable>
        <derivativeTransaction>
            <securityTitle>
                <value>Stock Options</value>
            </securityTitle>
            <conversionOrExercisePrice>
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                <value>2016-12-30</value>
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            <transactionTimeliness></transactionTimeliness>
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                    <value>75000</value>
                </transactionShares>
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            <exerciseDate>
                <value>2016-12-30</value>
                <footnoteId id="F6"/>
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            <expirationDate>
                <value>2025-12-29</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>75000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
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        <derivativeTransaction>
            <securityTitle>
                <value>Stock Options</value>
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                <value>0.88</value>
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                <value>2017-01-02</value>
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                <transactionFormType>4</transactionFormType>
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                <equitySwapInvolved>0</equitySwapInvolved>
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            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
                <transactionShares>
                    <value>125000</value>
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                <transactionPricePerShare>
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                    <value>A</value>
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            <exerciseDate>
                <value>2017-02-01</value>
                <footnoteId id="F6"/>
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            <expirationDate>
                <value>2027-01-01</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>125000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>125000</value>
                </sharesOwnedFollowingTransaction>
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            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeTransaction>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The 75,000 shares of Heat Biologics, Inc. (the &quot;Company&quot;) common stock, $0.0002 par value per share (the &quot;Common Stock&quot;), are represented by 75,000 restricted stock units (&quot;RSUs&quot;) granted under the Company's Amended and Restated 2014 Stock Incentive Plan (the &quot;Plan&quot;). Each RSU represents a contingent right to receive one share of Common Stock. The 75,000 RSUs will vest as follows: (i) 18,750 will vest on December 30, 2016; (ii) 18,750 will vest on December 30, 2017; (iii) 18,750 will vest on December 30, 2018; and (iv) 18,750 will vest on December 30, 2019, provided that the reporting person continues to serve as a member of the Board of Directors or otherwise is not terminated for cause prior to such dates.</footnote>
        <footnote id="F2">The 125,000 shares of Common Stock are represented by 125,000 RSUs granted under the Plan. Each RSU represents a contingent right to receive one share of Common Stock. The 125,000 RSUs will vest as follows: (i) 31,250 will vest on January 2, 2017; (ii) 31,250 will vest on January 2, 2018; (iii) 31,250 will vest on January 2, 2019; and (iv) 31,250 will vest on January 2, 2020, provided that the reporting person continues to serve as a member of the Board of Directors or otherwise is not terminated for cause prior to such dates.</footnote>
        <footnote id="F3">Held by Orion Holdings V, LLC (&quot;Orion&quot;). Mr. Wolf is managing member of Orion.</footnote>
        <footnote id="F4">Held by Seed-One Holdings VI, LLC (&quot;Seed-One&quot;). Mr. Wolf is managing member of Seed-One.</footnote>
        <footnote id="F5">Does not include 89,957 shares held by Mr. Wolf's children's trust (the &quot;Trust&quot;). Mr. Wolf is not the trustee, nor does he claim beneficial ownership of the Trust. Mr. Wolf disclaims beneficial ownership of the shares of Common Stock held by Orion, Seed-One and the Trust except to the extent of any pecuniary interest (as defined in Rule 16a-1(a)(2) promulgated under the Securities Exchange Act of 1934, as amended) that he may have in such entities.</footnote>
        <footnote id="F6">These stock options vest monthly on a pro rata basis over a four year period commencing February 1, 2017.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Jeffrey Alan Wolf</signatureName>
        <signatureDate>2017-01-04</signatureDate>
    </ownerSignature>
</ownershipDocument>
