<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3/A</documentType>

    <periodOfReport>2016-03-14</periodOfReport>

    <dateOfOriginalSubmission>2016-03-21</dateOfOriginalSubmission>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001476170</issuerCik>
        <issuerName>Unilife Corp</issuerName>
        <issuerTradingSymbol>UNIS</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001669700</rptOwnerCik>
            <rptOwnerName>Ratigan Michael</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>250 CROSS FARM LANE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>YORK</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>17406</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>See Remarks</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>49474</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Of these shares of common stock of Unilife Corporation (the &quot;Company&quot;), 8,224 shares were unintentionally omitted from the reporting person's original Form 3.  Of such omitted shares, 3,107 shares were awarded by the Company to the reporting person on April 14, 2014 and are scheduled to vest on April 30, 2017 subject to the reporting person's continued employment with the Company as of such date.  All share amounts reported herein reflect a 1-for-10 reverse stock split of the Company's common stock effected on May 13, 2016.</footnote>
    </footnotes>

    <remarks>SVP and Chief Commercial Officer</remarks>

    <ownerSignature>
        <signatureName>/s/ Kevin Campbell, as attorney-in-fact</signatureName>
        <signatureDate>2016-08-08</signatureDate>
    </ownerSignature>
</ownershipDocument>
