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          <cik>0001475940</cik>
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      <periodOfReport>09-30-2025</periodOfReport>
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      <isAmendment>false</isAmendment>
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        <name>MONTRUSCO BOLTON INVESTMENTS INC.</name>
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          <ns1:street1>1501 MCGILL COLLEGE AVENUE</ns1:street1>
          <ns1:street2>SUITE 1200</ns1:street2>
          <ns1:city>MONTREAL</ns1:city>
          <ns1:stateOrCountry>A8</ns1:stateOrCountry>
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      <name>Ginette Therien</name>
      <title>Senior VP, Governance and CCO</title>
      <phone>5142825417</phone>
      <signature>/s/ Ginette Therien</signature>
      <city>Montreal</city>
      <stateOrCountry>A8</stateOrCountry>
      <signatureDate>11-10-2025</signatureDate>
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