<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <!-- Generated by Workiva -->
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001996647</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001465740</issuerCik>
      <issuerName>Two Harbors Investment Corp.</issuerName>
      <secFileNumber>001-34506</secFileNumber>
      <issuerAddress>
        <com:street1>1601 Utica Avenue South</com:street1>
        <com:street2>Suite 100</com:street2>
        <com:city>St. Louis Park</com:city>
        <com:stateOrCountry>MN</com:stateOrCountry>
        <com:zipCode>55416</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>6124514100</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Nathan Boucher</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Raymond James</name>
        <address>
          <com:street1>660 Newport Center Drive</com:street1>
          <com:street2>Suite 570</com:street2>
          <com:city>Newport Beach</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>92660</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>2416</noOfUnitsSold>
      <aggregateMarketValue>25150.56</aggregateMarketValue>
      <noOfUnitsOutstanding>104155818</noOfUnitsOutstanding>
      <approxSaleDate>01/08/2026</approxSaleDate>
      <securitiesExchangeName>NYSE</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <acquiredDate>01/07/2026</acquiredDate>
      <natureOfAcquisitionTransaction>Vesting of Restricted Stock Unit Award</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Two Harbors Investment Corp.</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>1280</amountOfSecuritiesAcquired>
      <paymentDate>01/07/2026</paymentDate>
      <natureOfPayment>Vesting of Restricted Stock Unit Award</natureOfPayment>
    </securitiesToBeSold>
    <securitiesToBeSold>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <acquiredDate>01/08/2026</acquiredDate>
      <natureOfAcquisitionTransaction>Vesting of Restricted Stock Unit Award</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Two Harbors Investment Corp.</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>1136</amountOfSecuritiesAcquired>
      <paymentDate>01/08/2026</paymentDate>
      <natureOfPayment>Vesting of Restricted Stock Unit Award</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <remarks>The reporting person anticipates selling the shares for the purpose of satisfying income tax liabilities, incurred upon the vesting of restricted stock unit awards, pursuant to written instructions previously executed by the reporting person.</remarks>
    <noticeSignature>
      <noticeDate>01/07/2026</noticeDate>
      <planAdoptionDates>
        <planAdoptionDate>02/07/2024</planAdoptionDate>
      </planAdoptionDates>
      <signature>/s/ Nathan Boucher</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
