<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001464413</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


      <seriesClassInfo>
        <seriesId>S000038306</seriesId>
        <classId>C000118212</classId>
        <classId>C000118211</classId>
        <classId>C000200109</classId>
      </seriesClassInfo>


    </filerInfo>
  </headerData>
  <formData>
    <genInfo>
      <regName>Starboard Investment Trust</regName>
      <regFileNumber>811-22298</regFileNumber>
      <regCik>0001464413</regCik>
      <regLei>549300IGQEISLESO0528</regLei>
      <regStreet1>116 South Franklin Street</regStreet1>
      <regCity>Rocky Mount</regCity>
      <regStateConditional regCountry="US" regState="US-NC"/>
      <regZipOrPostalCode>27804</regZipOrPostalCode>
      <regPhone>252-972-9922</regPhone>
      <seriesName>Cavalier Tactical Rotation Fund</seriesName>
      <seriesId>S000038306</seriesId>
      <seriesLei>54930066MENKZJ5L2C64</seriesLei>
      <repPdEnd>2020-05-31</repPdEnd>
      <repPdDate>2020-05-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>21740911.12</totAssets>
      <totLiabs>1518775.12</totLiabs>
      <netAssets>20222136</netAssets>
      <assetsAttrMiscSec>0</assetsAttrMiscSec>
      <assetsInvested>0</assetsInvested>
      <amtPayOneYrBanksBorr>0</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0</amtPayAftOneYrOther>
      <delayDeliv>0</delayDeliv>
      <standByCommit>0</standByCommit>
      <liquidPref>0</liquidPref>
      <cshNotRptdInCorD>0</cshNotRptdInCorD>
      <curMetrics>
        <curMetric>
          <curCd>USD</curCd>
          <intrstRtRiskdv01 period10Yr="0" period1Yr="0" period30Yr="0" period3Mon="0" period5Yr="0.01"/>
          <intrstRtRiskdv100 period10Yr="0.49" period1Yr="0.19" period30Yr="0.01" period3Mon="0.06" period5Yr="1.21"/>
        </curMetric>
      </curMetrics>
      <creditSprdRiskInvstGrade period10Yr="0" period1Yr="81.75" period30Yr="0" period3Mon="71.24" period5Yr="464.12"/>
      <creditSprdRiskNonInvstGrade period10Yr="6293.7" period1Yr="828.66" period30Yr="183.94" period3Mon="139.22" period5Yr="9158.55"/>
      <borrowers>
        <borrower aggrVal="0" lei="N/A" name="N/A"/>
      </borrowers>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000118212" rtn1="-13.42" rtn2="3.95" rtn3="1.27"/>
        </monthlyTotReturns>
        <monthlyReturnCats>
          <equityContracts>
            <mon1 netRealizedGain="0" netUnrealizedAppr="0"/>
            <mon2 netRealizedGain="-241742.16" netUnrealizedAppr="-36575.45"/>
            <mon3 netRealizedGain="0" netUnrealizedAppr="-174125"/>
            <optionCategory>
              <instrMon1 netRealizedGain="0" netUnrealizedAppr="0"/>
              <instrMon2 netRealizedGain="-241742.16" netUnrealizedAppr="-36575.45"/>
              <instrMon3 netRealizedGain="0" netUnrealizedAppr="-174125"/>
            </optionCategory>
          </equityContracts>
        </monthlyReturnCats>
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        <othMon2 netRealizedGain="-507594.11" netUnrealizedAppr="1614004.1"/>
        <othMon3 netRealizedGain="66970.67" netUnrealizedAppr="382965.88"/>
      </returnInfo>
      <mon1Flow redemption="-3654654.28" reinvestment="0" sales="776952.01"/>
      <mon2Flow redemption="-915075.32" reinvestment="0" sales="548859.69"/>
      <mon3Flow redemption="-1318804.94" reinvestment="0" sales="686540.08"/>


    </fundInfo>
    <invstOrSecs>
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        <name>iShares Nasdaq Biote</name>
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        <title>ETF</title>
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        <identifiers>
          <isin value="US4642875565"/>
        </identifiers>
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        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>917175.18</valUSD>
        <pctVal>0.05</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Invesco QQQ Trust Se</name>
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          <isin value="US46090E1038"/>
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        <units>NS</units>
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        <valUSD>4379000.4</valUSD>
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        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Edge MSCI Mi</name>
        <lei>54930006KUZN6GLGL496</lei>
        <title>ETF</title>
        <cusip>46429B697</cusip>
        <identifiers>
          <isin value="US46429B6974"/>
        </identifiers>
        <balance>25818</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1590905.16</valUSD>
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        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vanguard Information</name>
        <lei>5493002789CX3L0CJP65</lei>
        <title>ETF</title>
        <cusip>92204A702</cusip>
        <identifiers>
          <isin value="US92204A7028"/>
        </identifiers>
        <balance>5890</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1537466.7</valUSD>
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        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Technology Select Se</name>
        <lei>549300BR5T0JNM2MW070</lei>
        <title>ETF</title>
        <cusip>81369Y803</cusip>
        <identifiers>
          <isin value="US81369Y8030"/>
        </identifiers>
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        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2798219.14</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Consumer Staples Sel</name>
        <lei>549300Z52QUV3UXHLA68</lei>
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        <cusip>81369Y308</cusip>
        <identifiers>
          <isin value="US81369Y3080"/>
        </identifiers>
        <balance>37114</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2198262.22</valUSD>
        <pctVal>0.11</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>VELOCITYSHARES 2X</name>
        <lei>549300ROEJDDAXM6LU05</lei>
        <title>ETN</title>
        <cusip>22542D258</cusip>
        <identifiers>
          <isin value="US22542D2586"/>
        </identifiers>
        <balance>19000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2697810</valUSD>
        <pctVal>0.13</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CH</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Fidelity Investments</name>
        <lei>6X064LF7Y6B4DKF2GZ26</lei>
        <title>MONEYMKT</title>
        <cusip>316175108</cusip>
        <identifiers>
          <other otherDesc="Ticker" value="FIGXX"/>
        </identifiers>
        <balance>5732996.4</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>5732996.4</valUSD>
        <pctVal>0.28</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Money Market" issuerCat="OTHER"/>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>SPDR S&amp;P 500 ETF Tru</name>
        <lei>549300NZAMSJ8FXPQQ63</lei>
        <title>OPTION</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="OCC Identifier" value="SPY 200529P00275000"/>
        </identifiers>
        <balance>350</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>175</valUSD>
        <pctVal>0</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerConditional desc="Option" issuerCat="OTHER"/>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <derivativeInfo>
          <optionSwaptionWarrantDeriv derivCat="OPT">
            <counterparties>
              <counterpartyName>INTERACT</counterpartyName>
              <counterpartyLei>50OBSE5T5521O6SMZR28</counterpartyLei>
            </counterparties>
            <putOrCall>Put</putOrCall>
            <writtenOrPur>Written</writtenOrPur>
            <descRefInstrmnt>
              <otherRefInst>
                <issuerName>SPDR S&amp;P 500 ETF Tru</issuerName>
                <issueTitle>ETF</issueTitle>
                <identifiers>
                  <ticker value="SPY"/>
                </identifiers>
              </otherRefInst>
            </descRefInstrmnt>
            <shareNo>0</shareNo>
            <exercisePrice>275</exercisePrice>
            <exercisePriceCurCd>USD</exercisePriceCurCd>
            <expDt>2020-05-30</expDt>
            <delta>XXXX</delta>
            <unrealizedAppr>-210700.45</unrealizedAppr>
          </optionSwaptionWarrantDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <signature>
      <ncom:dateSigned>2020-07-13</ncom:dateSigned>
      <ncom:nameOfApplicant>Starboard Investment Trust</ncom:nameOfApplicant>
      <ncom:signature>Ashley Lanham</ncom:signature>
      <ncom:signerName>Ashley Lanham</ncom:signerName>
      <ncom:title>Treasurer/PFO</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
