Exhibit 31.1

Certification of Chief Executive Officer

I, Mikkel Svane, Chief Executive Officer of Zendesk, Inc., certify that:

1. I have reviewed this Amendment No. 1 to the Annual Report on Form 10-K/A of Zendesk, Inc.; and

2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report.

Date: April 29, 2022

/s/ Mikkel Svane

Mikkel Svane

Chief Executive Officer