SCHEDULE 13G/A 0001104659-20-021154 0001697748 XXXXXXXX LIVE 6 Common Stock 09/30/2024 0001459417 2U, Inc. 90214J101 7900 Harkins Road Lanham MD 20706 Rule 13d-1(b) ARK Investment Management LLC DE 0.00 0.00 0.00 0.00 0.00 N 0.0 IA 2U, Inc. 7900 Harkins Road, Lanham, MD, 20706 ARK Investment Management LLC 200 Central Avenue, St. Petersburg, FL 33701 Delaware N IA 0 0.0 0 0 0 0 N Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. ARK Investment Management LLC /s/ Kellen Carter 01/10/2025