<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0704</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001453593</cik>
        <entityName>Bacterin International Holdings, Inc.</entityName>
        <issuerAddress>
            <street1>600 Cruiser Lane</street1>
            <city>Belgrade</city>
            <stateOrCountry>MT</stateOrCountry>
            <stateOrCountryDescription>MONTANA</stateOrCountryDescription>
            <zipCode>59714</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>406-388-0480</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <previousName>K-Kitz, Inc.</previousName>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <previousName>K KITZ INC</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2006</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Kent</firstName>
                <lastName>Swanson</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <stateOrCountryDescription>MONTANA</stateOrCountryDescription>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Michael</firstName>
                <lastName>Lopach</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <stateOrCountryDescription>MONTANA</stateOrCountryDescription>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Guy</firstName>
                <lastName>Cook</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <stateOrCountryDescription>MONTANA</stateOrCountryDescription>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>John</firstName>
                <lastName>Gandolfo</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 Cruiser Lane</street1>
                <city>Belgrade</city>
                <stateOrCountry>MT</stateOrCountry>
                <stateOrCountryDescription>MONTANA</stateOrCountryDescription>
                <zipCode>59714</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001144204-11-025737</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2011-04-19</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Middlebury Securities LLC</recipientName>
                <recipientCRDNumber>122602</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1043 Sheep Farm Road</street1>
                    <city>Weybridge</city>
                    <stateOrCountry>VT</stateOrCountry>
                    <stateOrCountryDescription>VERMONT</stateOrCountryDescription>
                    <zipCode>05753</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>VT</state>
                    <description>VERMONT</description>
                </statesOfSolicitationList>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>3500000</totalOfferingAmount>
            <totalAmountSold>3027504</totalAmountSold>
            <totalRemaining>472496</totalRemaining>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>true</hasNonAccreditedInvestors>
            <numberNonAccreditedInvestors>1</numberNonAccreditedInvestors>
            <totalNumberAlreadyInvested>16</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>20000</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Bacterin International Holdings, Inc.</issuerName>
                <signatureName>/s/ Guy Cook</signatureName>
                <nameOfSigner>Guy Cook</nameOfSigner>
                <signatureTitle>CEO</signatureTitle>
                <signatureDate>2011-05-31</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
