Exhibit 8.1

SUBSIDIARIES OF SEANERGY MARITIME HOLDINGS CORP.

Subsidiary

Jurisdiction of Incorporation

Seanergy Management Corp. Martinique International Corp. Harbour Business International Corp. Pembroke Chartering Services Limited Sea Glorius Shipping Co. Sea Genius Shipping Co. Seanergy Shipmanagement Corp. Leader Shipping Co. Premier Marine Co. Gladiator Shipping Co. Guardian Shipping Co. Fellow Shipping Co. Champion Ocean Navigation Co. Limited Squire Ocean Navigation Co. Maritime Capital Shipping Limited Maritime Capital Shipping (HK) Limited Maritime Glory Shipping Limited Maritime Grace Shipping Limited Atlantic Grace Shipping Limited Emperor Holding Ltd. Lord Ocean Navigation Co. Knight Ocean Navigation Co. Partner Shipping Co. Limited Fellow Shipping Co. Champion Marine Co. Champion Marine Co.

Marshall Islands British Virgin Islands British Virgin Islands Malta Marshall Islands Marshall Islands Marshall Islands Marshall Islands Marshall Islands Marshall Islands Marshall Islands Marshall Islands Malta Liberia Bermuda Hong Kong British Virgin Islands British Virgin Islands British Virgin Islands Marshall Islands Liberia Liberia Malta Marshall Islands Liberia Marshall Islands