<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3/A</documentType>

    <periodOfReport>2016-06-22</periodOfReport>

    <dateOfOriginalSubmission>2016-06-22</dateOfOriginalSubmission>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001447669</issuerCik>
        <issuerName>TWILIO INC</issuerName>
        <issuerTradingSymbol>TWLO</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001534338</rptOwnerCik>
            <rptOwnerName>Bessemer Venture Partners VII L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O BESSEMER VENTURE PARTNERS 1865</rptOwnerStreet1>
            <rptOwnerStreet2>PALMER AVE., SUITE 104</rptOwnerStreet2>
            <rptOwnerCity>LARCHMONT</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10538</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001540726</rptOwnerCik>
            <rptOwnerName>Deer VII &amp; Co. Ltd.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O BESSEMER VENTURE PARTNERS 1865</rptOwnerStreet1>
            <rptOwnerStreet2>PALMER AVE., SUITE 104</rptOwnerStreet2>
            <rptOwnerCity>LARCHMONT</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10538</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001540731</rptOwnerCik>
            <rptOwnerName>Deer VII &amp; Co. L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O BESSEMER VENTURE PARTNERS 1865</rptOwnerStreet1>
            <rptOwnerStreet2>PALMER AVE., SUITE 104</rptOwnerStreet2>
            <rptOwnerCity>LARCHMONT</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10538</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001459524</rptOwnerCik>
            <rptOwnerName>BVP VII SPECIAL OPPORTUNITY FUND LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O BESSEMER VENTURE PARTNERS 1865</rptOwnerStreet1>
            <rptOwnerStreet2>PALMER AVE., SUITE 104</rptOwnerStreet2>
            <rptOwnerCity>LARCHMONT</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10538</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001407577</rptOwnerCik>
            <rptOwnerName>Bessemer Venture Partners VII Institutional L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O BESSEMER VENTURE PARTNERS 1865</rptOwnerStreet1>
            <rptOwnerStreet2>PALMER AVE., SUITE 104</rptOwnerStreet2>
            <rptOwnerCity>LARCHMONT</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10538</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001677269</rptOwnerCik>
            <rptOwnerName>15 Angels LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O BESSEMER VENTURE PARTNERS 1865</rptOwnerStreet1>
            <rptOwnerStreet2>PALMER AVE., SUITE 104</rptOwnerStreet2>
            <rptOwnerCity>LARCHMONT</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10538</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
                <footnoteId id="F1"/>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>128375</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnotes</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Series A Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F4"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F4"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F4"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                    <footnoteId id="F1"/>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>436994</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnotes</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series B Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F5"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F5"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F5"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                    <footnoteId id="F1"/>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>8728534</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnotes</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F5"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series C Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F6"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F6"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F6"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                    <footnoteId id="F1"/>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>6025808</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnotes</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F6"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series D Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F7"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F7"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F7"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                    <footnoteId id="F1"/>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>5156216</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnotes</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F7"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series E Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F8"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F8"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F8"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                    <footnoteId id="F1"/>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>88417</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnotes</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F8"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Immediately prior to the closing of the Issuer's initial public offering and following the conversion of each series of the Issuer's convertible preferred stock into Common Stock, each share of Common Stock will be reclassified into one share of Class B Common Stock in an exempt transaction pursuant to Rule 16b-7.</footnote>
        <footnote id="F2">Deer VII &amp; Co. Ltd. (&quot;Deer Ltd.&quot;) is the general partner of Deer VII &amp; Co. L.P. (&quot;Deer L.P.&quot;), which is the general partner of Bessemer Venture Partners VII Institutional LP (&quot;BVP VII Inst&quot;), Bessemer Venture Partners VII, LP (&quot;BVP VII&quot;) and BVP VII Special Opportunity Fund LP.  15 Angels, LLC is  a wholly owned subsidiary of BVP VII Inst.   (&quot;BVP VII SOF&quot;, together with 15 Angels, LLC, BVP VII Inst and BVP VII, collectively, the &quot;Funds&quot;). Deer Ltd. and Deer L.P. disclaim beneficial ownership of the securities held by the Funds, and this report shall not be deemed an admission that Deer Ltd. and Deer L.P. are the beneficial owners of such securities, except to the extent of their pecuniary interest therein, if any, by virtue of their direct and indirect general partner interests in the Funds.</footnote>
        <footnote id="F3">As of the date hereof, BVP VII Inst, BVP VII and BVP VII SOF own 17,972 shares, 41,080 shares and 69,323 shares, respectively, of Common Stock.</footnote>
        <footnote id="F4">The Series A Preferred Stock is convertible into Common Stock on a 1:1 basis and has no expiration date. Immediately prior to the completion of the Issuer's initial public offering, all shares of Series A Preferred Stock will be converted into shares of Common Stock of the Issuer.As of the date hereof, 15 Angels LLC,  BVP VII Inst, BVP VII and BVP VII SOF own 399,702 shares, 5,220 shares, 11,934 shares and 20,138 shares, respectively, of Series A Preferred Stock.</footnote>
        <footnote id="F5">The Series B Preferred Stock is convertible into Common Stock on a 1:1 basis and has no expiration date. Immediately prior to the completion of the Issuer's initial public offering, all shares of Series B Preferred Stock will be converted into shares of Common Stock of the Issuer. As of the date hereof, BVP VII Inst, BVP VII and BVP VII SOF own 1,221,994 shares, 2,793,130 shares and 4,713,410 shares, respectively, of Series B Preferred Stock.</footnote>
        <footnote id="F6">The Series C Preferred Stock is convertible into Common Stock on a 1:1 basis and has no expiration date. Immediately prior to the completion of the Issuer's initial public offering, all shares of Series C Preferred Stock will be converted into shares of Common Stock of the Issuer. As of the date hereof, BVP VII Inst, BVP VII and BVP VII SOF own 843,614 shares, 1,928,258 shares and 3,253,936 shares, respectively, of Series C Preferred Stock.</footnote>
        <footnote id="F7">The Series D Preferred Stock is convertible into Common Stock on a 1:1 basis and has no expiration date. Immediately prior to the completion of the Issuer's initial public offering, all shares of Series D Preferred Stock will be converted into shares of Common Stock of the Issuer. As of the date hereof, BVP VII Inst, BVP VII and BVP VII SOF own 721,870  shares, 1,649,988 shares and 2,784,358 shares, respectively, of Series D Preferred Stock.</footnote>
        <footnote id="F8">The Series E Preferred Stock is convertible into Common Stock on a 1:1 basis and has no expiration date. Immediately prior to the completion of the Issuer's initial public offering, all shares of Series E  Preferred Stock will be converted into shares of Common Stock of the Issuer. As of the date hereof, BVP VII Inst, BVP VII and BVP VII SOF own 12,378  shares, 28,293 shares and 47,746 shares, respectively, of Series E Preferred Stock.</footnote>
    </footnotes>

    <remarks>This filing amends and restates the Form 3 filed by the Reporting Persons on 6/22/16 (accession number  0001104659-16-128875).  The original filing inadvertently omitted 15 Angels LLC from the list of Reporting Persons.  This filing adds 15 Angels LLC as a Reporting Person but is otherwise unchanged.</remarks>

    <ownerSignature>
        <signatureName>/s/ Scott Ring, General Counsel, Deer VII &amp; Co. Ltd., the General Partner of Deer VII &amp; Co. L.P., the General Partner of Bessemer Venture Partners VII L.P.</signatureName>
        <signatureDate>2016-06-23</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/Scott Ring, General Counsel, Deer VII &amp; Co. Ltd., the General Partner of Deer VII &amp; Co. L.P., the General Partner of Bessemer Venture Partners VII Institutional L.P.</signatureName>
        <signatureDate>2016-06-23</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/Scott Ring, General Counsel, Deer VII &amp; Co. Ltd., the General Partner of Deer VII &amp; Co. L.P., the General Partner of BVP VII Special Opportunity Fund L.P.</signatureName>
        <signatureDate>2016-06-23</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/Scott Ring, General Counsel, Deer VII &amp; Co. Ltd., the General Partner of Deer VII &amp; Co. L.P</signatureName>
        <signatureDate>2016-06-23</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Scott Ring, General Counsel, Deer VII &amp; Co. Ltd</signatureName>
        <signatureDate>2016-06-23</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Scott Ring, Authorized Person</signatureName>
        <signatureDate>2016-06-23</signatureDate>
    </ownerSignature>
</ownershipDocument>
