144 0001439716 XXXXXXXX LIVE 0001808805 NAUTILUS BIOTECHNOLOGY INC 001-39434 2701 Eastlake Avenue East Seattle WA 98102 (206) 333-2001 MARY GODWIN Officer Common Morgan Stanley Smith Barney LLC Executive Financial Services

1 New York Plaza 8th Floor New York NY 10004

38337 74262.60 125563965 12/17/2024 NASDAQ Common 12/17/2024 Stock Option Exercise ISSUER N 38337 12/17/2024 Cash N MARY GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 12/09/2024 35000 74588.50 MARY GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 12/03/2024 60000 131778.00 MARY GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 11/26/2024 60000 136512.00 MARY GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 11/21/2024 30000 70623.00 MARY GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 11/19/2024 30000 68046.00 MARY GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 11/11/2024 40000 98884.00 MARY ELIZABETH GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 11/08/2024 31078 78158.06 MARY ELIZABETH GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 11/07/2024 28922 79642.51 MARY ELIZABETH GODWIN

2701 Eastlake Avenue East Seattle WA 98102

Common 09/23/2024 47031 134739.11 12/17/2024 /s/ Mary Elizabeth Godwin