<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2017-09-15</periodOfReport>

    <issuer>
        <issuerCik>0001437491</issuerCik>
        <issuerName>Ecoark Holdings, Inc.</issuerName>
        <issuerTradingSymbol>EARK</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001227362</rptOwnerCik>
            <rptOwnerName>MEHRING PETER A</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>3333 S. PINNACLE HILLS PARKWAY</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 220</rptOwnerStreet2>
            <rptOwnerCity>ROGERS</rptOwnerCity>
            <rptOwnerState>AR</rptOwnerState>
            <rptOwnerZipCode>72758</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>true</isDirector>
            <isOfficer>false</isOfficer>
            <isTenPercentOwner>false</isTenPercentOwner>
            <isOther>false</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Ecoark Holdings, Inc.</value>
            </securityTitle>
            <transactionDate>
                <value>2017-09-15</value>
            </transactionDate>
            <deemedExecutionDate>
                <value>2017-09-15</value>
            </deemedExecutionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>F</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>43217</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>3.02</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1191258</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The transaction reported on this Form 4 DOES NOT REFLECT A SALE by the reporting person of any shares.The transaction reported on this Form 4 was mandated by the issuer's election under its 2013 Incentive Stock Plan to require the satisfaction of tax withholding obligations through a WITHHOLD TO COVER transaction.The transaction reported on this Form 4 DOES NOT REPRESENT A SALE or discretionary trade by the reporting person, but rather was effected for the limited purpose of satisfying tax withholding obligations upon a partial vesting of the restricted stock awarded to the reporting person under the Plan.</footnote>
    </footnotes>

    <remarks></remarks>

    <ownerSignature>
        <signatureName>/s/ PETER A MEHRING</signatureName>
        <signatureDate>2017-09-18</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>BY: TROY RICHARDS, ATTORNEY-IN-FACT</signatureName>
        <signatureDate>2017-09-18</signatureDate>
    </ownerSignature>
</ownershipDocument>
