0001426800 2025-08-07 2025-08-07 0001426800 1 2025-08-07 2025-08-07 0001426800 2 2025-08-07 2025-08-07 0001426800 3 2025-08-07 2025-08-07 0001426800 4 2025-08-07 2025-08-07 0001426800 5 2025-08-07 2025-08-07 0001426800 6 2025-08-07 2025-08-07 0001426800 7 2025-08-07 2025-08-07 iso4217:USD pure shares S-3 S-3MEF EX-FILING FEES 333-285970 0001426800 ASSEMBLY BIOSCIENCES, INC. N/A N/A 0.0001531 0.0001531 0.0001531 0.0001531 0.0001531 0.0001531 S-3 ASSEMBLY BIOSCIENCES, INC. Equity Common Stock, par value $0.001 per share true Equity Preferred Stock, par value $0.001 per share true Debt Debt Securities true Other Warrants true Other Subscription Rights true Other Units true false Unallocated (Universal) Shelf true 50000000.00 0.0001531 7655.00 50000000.00 7655.00 0.00 0.00 7655.00 The registrant previously registered the offer and sale of certain securities having a proposed maximum aggregate offering price of $250,000,000 pursuant to a Registration Statement on Form S-3 (File No. 333-285970) (the "Prior Registration Statement"), which was initially filed on March 20, 2025 and declared effective by the Securities and Exchange Commission on April 1, 2025. As of the date hereof, a balance of $250,000,000.00 of such securities remains unsold under the Prior Registration Statement. In accordance with Rule 462(b) under the Securities Act of 1933, as amended, and General Instruction IV(A) of Form S-3, the registrant is hereby registering the offer and sale of an additional $50,000,000.00 of its Securities. The additional amount of Securities that is being registered for offer and sale represents no more than 20% of the maximum aggregate offering price of the remaining securities available to be sold under the Prior Registration Statement.