Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in the following Registration Statements:

(1) Registration Statements (Form S-3 Nos. 333-212373, 333-214073 and 333-272718) of Iovance Biotherapeutics, Inc. and in the related Prospectuses,

(2) Registration Statement (Form S-8 No. 333-205097) pertaining to the 2011 Equity Incentive Plan of Iovance Biotherapeutics, Inc.,

(3) Registration Statement (Form S-8 No. 333-214567) pertaining to the 2014 Equity Incentive Plan of Iovance Biotherapeutics, Inc.,

(4) Registration Statement (Form S-8 No. 333-217638) pertaining to Maria Fardis RSUs of Iovance Biotherapeutics, Inc.,

(5) Registration Statements (Form S-8 Nos. 333-239316, 333-227242, 333-266544 and 333-272602) pertaining to the 2018 Equity Incentive Plan of Iovance Biotherapeutics, Inc.,

(6) Registration Statement (Form S-8 Nos. 333-239317 and 333-272601) pertaining to the 2020 Employee Stock Purchase Plan of Iovance Biotherapeutics, Inc., and

(7) Registration Statement (Form S-8 Nos. 333-259752, 333-263503 and 333-271810) pertaining to the 2021 Inducement Plan of Iovance Biotherapeutics, Inc.;

of our reports dated February 28, 2024, with respect to the consolidated financial statements of Iovance Biotherapeutics, Inc. and the effectiveness of internal control over financial reporting of Iovance Biotherapeutics, Inc. included in this Annual Report (Form 10-K) of Iovance Biotherapeutics, Inc. for the year ended December 31, 2023.

/s/ Ernst & Young LLP

San Mateo, California

February 28, 2024