X0306 4 2022-06-15 0 0001420565 AILERON THERAPEUTICS INC ALRN 0001709690 Ambros Reinhard J. C/O AILERON THERAPEUTICS, INC. 285 SUMMER STREET, SUITE 101 BOSTON MA 02210 1 0 0 0 Stock Option (right to buy) 0.397 2022-06-15 4 A 0 54500 0.00 A 2032-06-14 Common Stock 54500 54500 D This option was granted on June 15, 2022. The shares underlying the option are scheduled to vest in full on the earlier of (i) June 15, 2023 or (ii) the date of the Issuer's 2023 Annual Meeting of Stockholders. Exhibit Index: 24.1 Power of Attorney /s/ Manuel Aivado, attorney-in-fact for Reinhard J. Ambros 2022-06-16