<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001412665</cik>
        <entityName>MidWestOne Financial Group, Inc.</entityName>
        <issuerAddress>
            <street1>102 SOUTH CLINTON ST.</street1>
            <city>IOWA CITY</city>
            <stateOrCountry>IA</stateOrCountry>
            <stateOrCountryDescription>IOWA</stateOrCountryDescription>
            <zipCode>52240</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>319-356-5800</issuerPhoneNumber>
        <jurisdictionOfInc>IOWA</jurisdictionOfInc>
        <edgarPreviousNameList>
            <previousName>ISB Financial Corp.</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Charles</firstName>
                <middleName>N.</middleName>
                <lastName>Funk</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Executive Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Larry</firstName>
                <middleName>D.</middleName>
                <lastName>Albert</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Douglas</firstName>
                <middleName>H.</middleName>
                <lastName>Greeff</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Douglas</firstName>
                <middleName>K.</middleName>
                <lastName>True</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Richard</firstName>
                <middleName>R.</middleName>
                <lastName>Donohue</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Richard</firstName>
                <middleName>J.</middleName>
                <lastName>Hartig</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Nathaniel</firstName>
                <middleName>J.</middleName>
                <lastName>Kaeding</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ruth</firstName>
                <middleName>E.</middleName>
                <lastName>Stanoch</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Kurt</firstName>
                <middleName>R.</middleName>
                <lastName>Weise</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Janet</firstName>
                <middleName>E.</middleName>
                <lastName>Godwin</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Matthew</firstName>
                <middleName>J.</middleName>
                <lastName>Hayek</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Tracy</firstName>
                <middleName>S.</middleName>
                <lastName>McCormick</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Kevin</firstName>
                <middleName>W.</middleName>
                <lastName>Monson</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Barry</firstName>
                <middleName>S.</middleName>
                <lastName>Ray</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Senior Executive Vice President and Chief Financial Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>James</firstName>
                <middleName>M.</middleName>
                <lastName>Cantrell</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Senior Executive Vice President, Chief Investment Officer and Treasurer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gary</firstName>
                <middleName>L.</middleName>
                <lastName>Sims</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Executive Vice President and Chief Credit Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>David</firstName>
                <middleName>E.</middleName>
                <lastName>Lindstrom</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Executive Vice President, Retail Banking of the Issuer's banking subsidiary</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gregory</firstName>
                <middleName>W.</middleName>
                <lastName>Turner</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Vice President, Wealth Management and Corporate Communications</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Len</firstName>
                <middleName>D.</middleName>
                <lastName>Devaisher</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>President and Chief Operating Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Jennifer</firstName>
                <middleName>L.</middleName>
                <lastName>Hauschildt</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>102 S. Clinton Street</street1>
                <city>Iowa City</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>52240</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Commercial Banking</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2020-07-28</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isDebtType>true</isDebtType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>100000</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Piper Sandler &amp; Co.</recipientName>
                <recipientCRDNumber>665</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>800 Nicollet Mall</street1>
                    <city>Minneapolis</city>
                    <stateOrCountry>MN</stateOrCountry>
                    <stateOrCountryDescription>MINNESOTA</stateOrCountryDescription>
                    <zipCode>55402</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>AL</state>
                    <description>ALABAMA</description>
                    <state>AR</state>
                    <description>ARKANSAS</description>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>CT</state>
                    <description>CONNECTICUT</description>
                    <state>FL</state>
                    <description>FLORIDA</description>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>IL</state>
                    <description>ILLINOIS</description>
                    <state>IN</state>
                    <description>INDIANA</description>
                    <state>IA</state>
                    <description>IOWA</description>
                    <state>KS</state>
                    <description>KANSAS</description>
                    <state>KY</state>
                    <description>KENTUCKY</description>
                    <state>MD</state>
                    <description>MARYLAND</description>
                    <state>MA</state>
                    <description>MASSACHUSETTS</description>
                    <state>MN</state>
                    <description>MINNESOTA</description>
                    <state>MS</state>
                    <description>MISSISSIPPI</description>
                    <state>MO</state>
                    <description>MISSOURI</description>
                    <state>MT</state>
                    <description>MONTANA</description>
                    <state>NE</state>
                    <description>NEBRASKA</description>
                    <state>NC</state>
                    <description>NORTH CAROLINA</description>
                    <state>ND</state>
                    <description>NORTH DAKOTA</description>
                    <state>OH</state>
                    <description>OHIO</description>
                    <state>OK</state>
                    <description>OKLAHOMA</description>
                    <state>PA</state>
                    <description>PENNSYLVANIA</description>
                    <state>SD</state>
                    <description>SOUTH DAKOTA</description>
                    <state>TX</state>
                    <description>TEXAS</description>
                    <state>VA</state>
                    <description>VIRGINIA</description>
                    <state>WA</state>
                    <description>WASHINGTON</description>
                    <state>WI</state>
                    <description>WISCONSIN</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>D.A. Davidson &amp; Co.</recipientName>
                <recipientCRDNumber>199</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>8 Third Street North</street1>
                    <city>Great Falls</city>
                    <stateOrCountry>MT</stateOrCountry>
                    <stateOrCountryDescription>MONTANA</stateOrCountryDescription>
                    <zipCode>59401</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>AZ</state>
                    <description>ARIZONA</description>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>IL</state>
                    <description>ILLINOIS</description>
                    <state>IN</state>
                    <description>INDIANA</description>
                    <state>IA</state>
                    <description>IOWA</description>
                    <state>MD</state>
                    <description>MARYLAND</description>
                    <state>MO</state>
                    <description>MISSOURI</description>
                    <state>MT</state>
                    <description>MONTANA</description>
                    <state>NE</state>
                    <description>NEBRASKA</description>
                    <state>NY</state>
                    <description>NEW YORK</description>
                    <state>ND</state>
                    <description>NORTH DAKOTA</description>
                    <state>PA</state>
                    <description>PENNSYLVANIA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>Janney Montgomery Scott LLC</recipientName>
                <recipientCRDNumber>463</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1717 Arch Street</street1>
                    <city>Philadelphia</city>
                    <stateOrCountry>PA</stateOrCountry>
                    <stateOrCountryDescription>PENNSYLVANIA</stateOrCountryDescription>
                    <zipCode>19103</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>CO</state>
                    <description>COLORADO</description>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>IL</state>
                    <description>ILLINOIS</description>
                    <state>IN</state>
                    <description>INDIANA</description>
                    <state>IA</state>
                    <description>IOWA</description>
                    <state>MN</state>
                    <description>MINNESOTA</description>
                    <state>MO</state>
                    <description>MISSOURI</description>
                    <state>NY</state>
                    <description>NEW YORK</description>
                    <state>OH</state>
                    <description>OHIO</description>
                    <state>VA</state>
                    <description>VIRGINIA</description>
                    <state>WI</state>
                    <description>WISCONSIN</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>65000000</totalOfferingAmount>
            <totalAmountSold>65000000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>33</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>975000</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>MidWestOne Financial Group, Inc.</issuerName>
                <signatureName>Barry S. Ray</signatureName>
                <nameOfSigner>Barry S. Ray</nameOfSigner>
                <signatureTitle>Senior Executive Vice President and CFO</signatureTitle>
                <signatureDate>2020-08-10</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
