X0306 4 2022-05-13 0 0001411690 Bionano Genomics, Inc. BNGO 0001822991 Stewart Christopher P. C/O BIONANO GENOMICS, INC. 9540 TOWNE CENTRE DRIVE, SUITE 100 SAN DIEGO CA 92121 0 1 0 0 Chief Financial Officer Common Stock 2022-05-13 4 M 0 60000 0.56 A 121579 D Stock Option (Right to Buy) 0.56 2022-05-13 4 M 0 60000 0.00 D 2030-09-01 Common Stock 60000 117500 D Includes 3,139 shares acquired under the Issuer's 2018 Employee Stock Purchase Plan (the "ESPP") on June 10, 2021 and 2,940 shares acquired under the ESPP on December 10, 2021. The shares vest in full on December 31, 2020, subject to Reporting Person's continuous service through December 31, 2020, subject to acceleration of vesting provided in the Employment Agreement, dated as of September 1, 2020, by and between the Issuer and the Reporting Person. /s/ Christopher P. Stewart 2022-05-16