X0306 4 2021-05-12 0 0001411690 Bionano Genomics, Inc BNGO 0001579988 Holmlin R. Erik C/O BIONANO GENOMICS, INC. 9540 TOWNE CENTRE DRIVE, SUITE 100 SAN DIEGO CA 92121 1 1 0 0 President and CEO Common Stock 2021-05-12 4 A 0 290000 0.00 A 295191 D Common Stock 2021-05-12 4 A 0 290000 0.00 A 585191 D Represents restricted stock unit award granted under the Issuer's 2018 Equity Incentive Plan. Includes: 218 shares acquired under the Issuer's 2018 Employee Stock Purchase Plan (the "ESPP") on December 10, 2018, 450 shares acquired under the ESPP on June 10, 2019, 728 shares acquired under the ESPP on December 10, 2019, 1,014 shares acquired under the ESPP on June 10, 2020 and 1,151 shares acquired under the ESPP on December 10, 2020. /s/ R. Erik Holmlin 2021-05-14