<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <!-- Generated by Workiva -->
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001405290</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001937993</issuerCik>
      <issuerName>CADRENAL THERAPEUTICS, INC.</issuerName>
      <secFileNumber>001-41596</secFileNumber>
      <issuerAddress>
        <com:street1>822 A1A NORTH</com:street1>
        <com:street2>SUITE 306</com:street2>
        <com:city>PONTE VEDRA BEACH</com:city>
        <com:stateOrCountry>FL</com:stateOrCountry>
        <com:zipCode>32082</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>904-300-0701</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Quang X. Pham</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Merrill Lynch</name>
        <address>
          <com:street1>824 A1A North</com:street1>
          <com:street2>Suite 200</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>1315</noOfUnitsSold>
      <aggregateMarketValue>18396.86</aggregateMarketValue>
      <noOfUnitsOutstanding>2046854</noOfUnitsOutstanding>
      <approxSaleDate>10/27/2025</approxSaleDate>
      <securitiesExchangeName>NASDAQ</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>01/25/2022</acquiredDate>
      <natureOfAcquisitionTransaction>Founders Shares</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>1315</amountOfSecuritiesAcquired>
      <paymentDate>01/25/2022</paymentDate>
      <natureOfPayment>N/A</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>08/27/2025</saleDate>
      <amountOfSecuritiesSold>3836</amountOfSecuritiesSold>
      <grossProceeds>53455.56</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>08/28/2025</saleDate>
      <amountOfSecuritiesSold>1193</amountOfSecuritiesSold>
      <grossProceeds>16540.64</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>09/24/2025</saleDate>
      <amountOfSecuritiesSold>2581</amountOfSecuritiesSold>
      <grossProceeds>36108</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>09/25/2025</saleDate>
      <amountOfSecuritiesSold>6790</amountOfSecuritiesSold>
      <grossProceeds>95661.94</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>09/29/2025</saleDate>
      <amountOfSecuritiesSold>419</amountOfSecuritiesSold>
      <grossProceeds>5869.5</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>10/01/2025</saleDate>
      <amountOfSecuritiesSold>881</amountOfSecuritiesSold>
      <grossProceeds>12343.19</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>10/02/2025</saleDate>
      <amountOfSecuritiesSold>500</amountOfSecuritiesSold>
      <grossProceeds>7066</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>10/09/2025</saleDate>
      <amountOfSecuritiesSold>1800</amountOfSecuritiesSold>
      <grossProceeds>25227.76</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>10/13/2025</saleDate>
      <amountOfSecuritiesSold>1800</amountOfSecuritiesSold>
      <grossProceeds>25470.4</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Quang X. Pham</name>
        <address>
          <com:street1>830 A1A North</com:street1>
          <com:street2>Suite 196</com:street2>
          <com:city>Ponte Vedra Beach</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>32082</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>10/24/2025</saleDate>
      <amountOfSecuritiesSold>1129</amountOfSecuritiesSold>
      <grossProceeds>15840.87</grossProceeds>
    </securitiesSoldInPast3Months>
    <noticeSignature>
      <noticeDate>10/27/2025</noticeDate>
      <planAdoptionDates>
        <planAdoptionDate>05/09/2025</planAdoptionDate>
      </planAdoptionDates>
      <signature>Quang X. Pham</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
