X0202 SCHEDULE 13G/A 0000935836-26-000109 0001105806 XXXXXXXX LIVE 3 Common Stock 06/30/2026 0001385818 AYTU BIOPHARMA, INC 054754858 7900 E. UNION AVENUE SUITE 920 DENVER CO 80237 Rule 13d-1(c) LYTTON LAURENCE W X1 0.00 545946.00 0.00 545946.00 545946.00 N 4.99 IN The shares of the Common Stock reported herein consist of 338,344 shares of Common Stock and warrants to purchase 731,666 shares of Common Stock, subject to a 4.99% beneficial ownership limitation. The percentages reported in this Schedule 13G are based on 10,733,208 shares of Common Stock outstanding as of April 30, 2026, as reported in the Form 10-Q filed by the Issuer for the quarterly period ended March 31, 2026. LYTTON-KAMBARA FOUNDATION X1 0.00 545946.00 0.00 545946.00 545946.00 N 4.99 IN The shares of the Common Stock reported herein consist of 338,344 shares of Common Stock and warrants to purchase 398,310 shares of Common Stock, subject to a 4.99% beneficial ownership limitation. The percentages reported in this Schedule 13G are based on 10,733,208 shares of Common Stock outstanding as of April 30, 2026, as reported in the Form 10-Q filed by the Issuer for the quarterly period ended March 31, 2026. AYTU BIOPHARMA, INC 7900 E. UNION AVENUE, SUITE 920, DENVER, CO, 80237 Laurence W. Lytton and Lytton-Kambara Foundation ("Foundation") 467 Central Park West New York, NY 10025 USA Y Lytton: 545,946 Foundation: 545,946 Lytton: 4.99% Foundation: 4.99% Lytton: 0 Foundation: 0 Lytton: 545,946 Foundation: 545,946 Lytton: 0 Foundation: 0 Lytton: 545,946 Foundation: 545,946 N Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under § 240.14a-11. EXHIBIT 99.1 - AGREEMENT REGARDING JOINT FILING OF STATEMENT ON SCHEDULE 13D OR 13G LYTTON LAURENCE W /s/ Laurence W. Lytton 08/13/2026 LYTTON-KAMBARA FOUNDATION /s/ Laurence W. Lytton 08/13/2026