<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2022-08-08</periodOfReport>

    <issuer>
        <issuerCik>0001379006</issuerCik>
        <issuerName>NANOVIRICIDES, INC.</issuerName>
        <issuerTradingSymbol>NNVC</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001513285</rptOwnerCik>
            <rptOwnerName>Diwan Anil</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NANOVIRICIDES, INC.,</rptOwnerStreet1>
            <rptOwnerStreet2>1 CONTROL DRIVE</rptOwnerStreet2>
            <rptOwnerCity>SHELTON</rptOwnerCity>
            <rptOwnerState>CT</rptOwnerState>
            <rptOwnerZipCode>06484</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>true</isDirector>
            <isOfficer>true</isOfficer>
            <officerTitle>President</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock, par value $0.001 per share</value>
            </securityTitle>
            <transactionDate>
                <value>2022-08-08</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>G</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>94471</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The reported transaction involves a gift made by the reporting person for estate planning purposes to an irrevocable trust whose beneficiaries are his minor children and of which he is neither the trustee nor a beneficiary.</footnote>
        <footnote id="F2">Does not include 6,074 shares of Series A Convertible Preferred Stock (the &quot;Series A Stock&quot;) owned by the reporting person which is convertible into three and one-half shares of common stock upon limited conditions.</footnote>
        <footnote id="F3">Does not include 200,000 shares of Series A Stock or 470,959 shares of common stock owned by TheraCour Pharma, Inc., over which the reporting person holds voting and dispositive power.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Anil Diwan</signatureName>
        <signatureDate>2022-08-10</signatureDate>
    </ownerSignature>
</ownershipDocument>
