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  <headerData>
    <submissionType>13F-HR</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <flags>
        <confirmingCopyFlag>false</confirmingCopyFlag>
        <returnCopyFlag>false</returnCopyFlag>
        <overrideInternetFlag>false</overrideInternetFlag>
      </flags>
      <filer>
        <credentials>
          <cik>0001369913</cik>
          <ccc>XXXXXXXX</ccc>
        </credentials>
      </filer>
      <periodOfReport>12-31-2016</periodOfReport>
    </filerInfo>
  </headerData>
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    <coverPage>
      <reportCalendarOrQuarter>12-31-2016</reportCalendarOrQuarter>
      <filingManager>
        <name>American Investment Services, Inc.</name>
        <address>
          <ns1:street1>250 DIVISION STREET</ns1:street1>
          <ns1:city>GREAT BARRINGTON</ns1:city>
          <ns1:stateOrCountry>MA</ns1:stateOrCountry>
          <ns1:zipCode>01230</ns1:zipCode>
        </address>
      </filingManager>
      <reportType>13F HOLDINGS REPORT</reportType>
      <form13FFileNumber>028-12178</form13FFileNumber>
      <provideInfoForInstruction5>N</provideInfoForInstruction5>
    </coverPage>
    <signatureBlock>
      <name>Seth L. Hoffman</name>
      <title>Vice President</title>
      <phone>413 528-1216</phone>
      <signature>/s/  Seth L. Hoffman</signature>
      <city>Great Barrington</city>
      <stateOrCountry>MA</stateOrCountry>
      <signatureDate>02-09-2017</signatureDate>
    </signatureBlock>
    <summaryPage>
      <otherIncludedManagersCount>0</otherIncludedManagersCount>
      <tableEntryTotal>101</tableEntryTotal>
      <tableValueTotal>239454</tableValueTotal>
      <isConfidentialOmitted>false</isConfidentialOmitted>
    </summaryPage>
  </formData>
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