Exhibit 21.1

HEALTHCARE TRUST OF AMERICA, INC.

SUBSIDIARIES

Healthcare Management of America, Inc. (Delaware)

Healthcare Trust of America Holdings, LP (Delaware)

HTA - 1092 Madison, LLC (Delaware)

HTA - 1223 Washington, LLC (Delaware)

HTA - 13020 Telecom, LLC (Delaware)

HTA - 2750 Monroe, LLC (Delaware)

HTA - 5995 Plaza Drive, LLC (Delaware)

HTA - Academy, LLC (Delaware)

HTA - Amarillo Hospital, LLC (Delaware)

HTA - Augusta SS Hospital, LLC (Delaware)

HTA - Bayview, LLC (Delaware)

HTA - Bonnie Brae, LLC (Delaware)

HTA - Bristol West, LLC (Delaware)

HTA - Camp Creek III, LLC (Delaware)

HTA - Camp Creek, LLC (Delaware)

HTA - Cannon Park Place, LLC (Delaware)

HTA - Carney MOB, LLC (Delaware)

HTA - Chesterfield Rehab Hospital, LLC (Delaware)

HTA - Commons V, LLC (Delaware)

HTA - Corsicana, LLC (Delaware)

HTA - County Line Road, LLC (Delaware)

HTA - Crawfordsville, LLC (Delaware)

HTA - Cypress Station, LLC (Delaware)

HTA - Dallas LTAC, LLC (Delaware)

HTA - Dallas SS Hospital, LLC (Delaware)

HTA - Decatur Medical Plaza, LLC (Delaware)

HTA - Denton, LLC (Delaware)

HTA - Des Peres, LLC (Delaware)

HTA - Desert Ridge, LLC (Delaware)

HTA - Duke Chesterfield Rehab, LLC (Delaware)

HTA - E Florida LTC, LLC (Delaware)

HTA - East Cooper, LLC (Delaware)

HTA - Epler Parke Building B, LLC (Delaware)

HTA - Evansville Annex, LLC (Delaware)

HTA - Evansville Fourth, LLC (Delaware)

HTA - Evansville Gateway, LLC (Delaware)

HTA - Evansville Main, LLC (Delaware)

HTA - Evansville Westside, LLC (Delaware)

HTA - Fannin LP, LLC (Delaware)

HTA - Fannin, LLC (Delaware)

HTA - Federal North MOB, LLC (Delaware)

HTA - FL Ortho Institute ASC, LLC (Delaware)

HTA - Fort Road Medical, LLC (Delaware)

HTA - Fort Wayne, LLC (Delaware)

HTA - FP Pavillion, LLC (Delaware)

HTA - Gallery Medical, LLC (Delaware)

HTA - Gateway Tucson, LLC (Delaware)

HTA - Good Sam Cancer Center, LLC (Delaware)

HTA - Good Sam MOB, LLC (Delaware)

HTA - Greenville CFM, LLC (Delaware)

HTA - Greenville CHOS, LLC (Delaware)

HTA - Greenville Cleveland Street, LLC (Delaware)

HTA - Greenville Greer MOB A, LLC (Delaware)

HTA - Greenville Greer MOB B, LLC (Delaware)

HTA - Greenville Life Center, LLC (Delaware)

HTA - Greenville Maxwell Pointe, LLC (Delaware)

HTA - Greenville Memorial CTC, LLC (Delaware)

HTA - Greenville Memorial ISB, LLC (Delaware)

HTA - Greenville Memorial MOB, LLC (Delaware)

HTA - Greenville Mills Avenue, LLC (Delaware)

HTA - Greenville Patewood Administration, LLC (Delaware)

HTA - Greenville Patewood MOB A, LLC (Delaware)

HTA - Greenville Patewood MOB B, LLC (Delaware)

HTA - Greenville Patewood MOB C, LLC (Delaware)

HTA - Greenville Travelers Rest, LLC (Delaware)

HTA - Greenville, LLC (Delaware)

HTA - Gwinnett, LLC (Delaware)

HTA - Hampden Place MOB (Delaware)

HTA - Hilton Head, LLC (Delaware)

HTA - Holy Family MOB, LLC (Delaware)

HTA - Jackson's Row, LLC (Delaware)

HTA - King Street, LLC (Delaware)

HTA - Kokomo Medical Office Park, LLC (Delaware)

HTA - Lenox Medical, LLC (Delaware)

HTA - Lewisville MOB, LLC (Delaware)

HTA - Liberty Falls Medical Plaza, LLC (Delaware)

HTA - Lima, LLC (Delaware)

HTA - Lima-207, LLC (Delaware)

HTA - Lincoln Park Boulevard, LLC (Delaware)

HTA - Loch Raven, LLC (Delaware)

HTA - Maple Avenue Investor, LLC (Delaware)

HTA - Maple Avenue, LLC (Delaware)

HTA - Marietta Health Park, LLC (Delaware)

HTA - Market Exchange, LLC (Delaware)

HTA - Mary Black MOB (Delaware)

HTA - Medical Portfolio 1, LLC (Delaware)

HTA - Medical Portfolio 2, LLC (Delaware)

HTA - Medical Portfolio 2-St. Louis, LLC (Delaware)

HTA - Medical Portfolio 3, LLC (Delaware)

HTA - Medical Portfolio 4, LLC (Delaware)

HTA - Medical Portfolio 4-Parma, LLC (Delaware)

HTA - Medical Portfolio 4-Phoenix, LLC (Delaware)

HTA - Morton MOB, LLC (Delaware)

HTA - Moss Creek, LLC (Delaware)

HTA - Mountain Empire, LLC (Delaware)

HTA - Mountain Plains-CO, LLC (Delaware)

HTA - Mountain Plains-TX, LLC (Delaware)

HTA - MPOC, LLC (Delaware)

HTA - Nashoba MOB 1, LLC (Delaware)

HTA - Nashoba MOB 2, LLC (Delaware)

HTA - Northern Berkshire, LLC (Delaware)

HTA - Northmeadow, LLC (Delaware)

HTA - Norwood Cancer Center, LLC (Delaware)

HTA - Norwood MOB, LLC (Delaware)

HTA - Nutfield Professional Center, LLC (Delaware)

HTA - Oklahoma City, LLC (Delaware)

HTA - Orlando SS Hospital, LLC (Delaware)

HTA - Overlook, LLC (Delaware)

HTA - Oviedo, LLC (Delaware)

HTA - Patroon Creek, LLC (Delaware)

HTA - Peachtree, LLC (Delaware)

HTA - Pearland Broadway, LLC (Delaware)

HTA - Pearland Cullen, LLC (Delaware)

HTA - Penn Ave, LLC (Delaware)

HTA - Phoenix Estrella, LLC (Delaware)

HTA - Phoenix Medical Center, LLC (Delaware)

HTA - Phoenix Paseo, LLC (Delaware)

HTA - Plan B - MOB, LP (Texas)

HTA - Poughkeepsie, LLC (Delaware)

HTA - Presidential, LLC (Delaware)

HTA - Putnam Center, LLC (Delaware)

HTA - Raleigh, LLC (Delaware)

HTA - Region Health, LLC (Delaware)

HTA - Renaissance, LLC (Delaware)

HTA - Rush, LLC (Delaware)

HTA - San Angelo, LLC (Delaware)

HTA - San Martin, LLC (Delaware)

HTA - Santa Fe 1640, LLC (Delaware)

HTA - Santa Fe 440, LLC (Delaware)

HTA - Sapling Grove, LLC (Delaware)

HTA - SC 13041 DWB, LLC (Delaware)

HTA - SC Boswell Medical, LLC (Delaware)

HTA - SC Boswell West, LLC (Delaware)

HTA - SC Cardiac Care, LLC (Delaware)

HTA - SC Eye Institute, LLC (Delaware)

HTA - SC Lakes Club, LLC (Delaware)

HTA - SC Lakes Medical Plaza I, LLC (Delaware)

HTA - SC Lakeview Medical Arts, LLC (Delaware)

HTA - SC Lakeview Plaza Centre, LLC (Delaware)

HTA - SC Royal Oaks, LLC (Delaware)

HTA - SCW Colonnade, LLC (Delaware)

HTA - SCW Granite Valley MOB, LLC (Delaware)

HTA - SCW Mountain View, LLC (Delaware)

HTA - SCW Webb Medical A, LLC (Delaware)

HTA - SCW Webb Medical B, LLC (Delaware)

HTA - SCW Webb Medical C, LLC (Delaware)

HTA - SCW West Medical Arts, LLC (Delaware)

HTA - Senior Care Portfolio 1, LLC (Delaware)

HTA - Sierra Vista, LLC (Delaware)

HTA - SJ Providence, LLC (Delaware)

HTA - Skylyn, LLC (Delaware)

HTA - Southcrest, LLC (Delaware)

HTA - Southpointe, LLC (Delaware)

HTA - St. Annes MOB 1, LLC (Delaware)

HTA - St. Annes MOB 2, LLC (Delaware)

HTA - St. Elizabeths MOB 1, LLC (Delaware)

HTA - St. Elizabeths MOB 2, LLC (Delaware)

HTA - St. Mary Physician Center, LLC (Delaware)

HTA - Sugar Land, LLC (Delaware)

HTA - Tallahassee SS Hospital, LLC (Delaware)

HTA - Thunderbird Medical, LLC (Delaware)

HTA - Triad, LLC (Delaware)

HTA - Triumph, LLC (Delaware)

HTA - Tucson Medical Office, LLC (Delaware)

HTA - Underhill, LLC (Delaware)

HTA - Vista Professional Center, LLC (Delaware)

HTA - Washington Medical Arts I Fee, LLC (Delaware)

HTA - Washington Medical Arts I, LLC (Delaware)

HTA - Washington Medical Arts II Fee, LLC (Delaware)

HTA - Washington Medical Arts II, LLC (Delaware)

HTA - Wellington, LLC (Delaware)

HTA - Wisconsin MOB 2, LLC (Delaware)

HTA - Wisconsin MOB Portfolio, LLC (Delaware)

HTA Tenant Services TRS, Inc. (Delaware)

NNN Lenox Medical Land, LLC (Delaware)