<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001355064</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>
      <seriesClassInfo>
        <seriesId>S000041894</seriesId>
        <classId>C000130116</classId>
        <classId>C000130115</classId>
        <classId>C000130114</classId>
      </seriesClassInfo>

    </filerInfo>
  </headerData>
  <formData>
    <genInfo>
      <regName>MUTUAL FUND SERIES TRUST</regName>
      <regFileNumber>811-21872</regFileNumber>
      <regCik>0001355064</regCik>
      <regLei>5493002ZGLQMLR4QMA96</regLei>
      <regStreet1>C/O GEMINI FUND SERVICES LLC</regStreet1>
      <regStreet2>4221 North 203rd Street, Suite 100</regStreet2>
      <regCity>ELKHORN</regCity>
      <regStateConditional regCountry="US" regState="US-NE"/>
      <regZipOrPostalCode>68022</regZipOrPostalCode>
      <regPhone>631-470-2600</regPhone>
      <seriesName>Catalyst/Warrington Strategic Program Fund</seriesName>
      <seriesId>S000041894</seriesId>
      <seriesLei>549300HQL3WT31ZF0202</seriesLei>
      <repPdEnd>2022-06-30</repPdEnd>
      <repPdDate>2021-12-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>105049010.13</totAssets>
      <totLiabs>244483.58</totLiabs>
      <netAssets>104804526.55</netAssets>
      <assetsAttrMiscSec>0.00000000</assetsAttrMiscSec>
      <assetsInvested>0.00000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.00000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.00000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.00000000</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0.00000000</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0.00000000</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0.00000000</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0.00000000</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0.00000000</amtPayAftOneYrOther>
      <delayDeliv>0.00000000</delayDeliv>
      <standByCommit>0.00000000</standByCommit>
      <liquidPref>0.00000000</liquidPref>
      <cshNotRptdInCorD>19221558.75000000</cshNotRptdInCorD>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000130116" rtn1="0.36000000" rtn2="0.96000000" rtn3="1.19000000"/>
          <monthlyTotReturn classId="C000130115" rtn1="0.26000000" rtn2="0.92000000" rtn3="1.04000000"/>
          <monthlyTotReturn classId="C000130114" rtn1="0.37000000" rtn2="0.86000000" rtn3="1.22000000"/>
        </monthlyTotReturns>
        <monthlyReturnCats>
          <equityContracts>
            <mon1 netRealizedGain="661673.20000000" netUnrealizedAppr="-59450.00000000"/>
            <mon2 netRealizedGain="799881.24000000" netUnrealizedAppr="369512.50000000"/>
            <mon3 netRealizedGain="1753980.00000000" netUnrealizedAppr="-316212.50000000"/>
            <futureCategory>
              <instrMon1 netRealizedGain="0.00000000" netUnrealizedAppr="0.00000000"/>
              <instrMon2 netRealizedGain="0.00000000" netUnrealizedAppr="0.00000000"/>
              <instrMon3 netRealizedGain="717500.00000000" netUnrealizedAppr="0.00000000"/>
            </futureCategory>
            <optionCategory>
              <instrMon1 netRealizedGain="661673.20000000" netUnrealizedAppr="-59450.00000000"/>
              <instrMon2 netRealizedGain="799881.24000000" netUnrealizedAppr="369512.50000000"/>
              <instrMon3 netRealizedGain="1036480.00000000" netUnrealizedAppr="-316212.50000000"/>
            </optionCategory>
          </equityContracts>
          <otherContracts>
            <mon1 netRealizedGain="-65467.56000000" netUnrealizedAppr="0.00000000"/>
            <mon2 netRealizedGain="-128901.47000000" netUnrealizedAppr="0.00000000"/>
            <mon3 netRealizedGain="-64068.06000000" netUnrealizedAppr="0.00000000"/>
            <futureCategory>
              <instrMon1 netRealizedGain="-65467.56000000" netUnrealizedAppr="0.00000000"/>
              <instrMon2 netRealizedGain="-128901.47000000" netUnrealizedAppr="0.00000000"/>
              <instrMon3 netRealizedGain="-64068.06000000" netUnrealizedAppr="0.00000000"/>
            </futureCategory>
          </otherContracts>
        </monthlyReturnCats>
        <othMon1 netRealizedGain="183194.95000000" netUnrealizedAppr="-150538.30000000"/>
        <othMon2 netRealizedGain="195757.50000000" netUnrealizedAppr="-172778.84000000"/>
        <othMon3 netRealizedGain="99789.05000000" netUnrealizedAppr="-65246.50000000"/>
      </returnInfo>
      <mon1Flow redemption="-2026050.84000000" reinvestment="0.00000000" sales="1104186.67000000"/>
      <mon2Flow redemption="-2062738.42000000" reinvestment="0.00000000" sales="1288839.15000000"/>
      <mon3Flow redemption="-2399354.66000000" reinvestment="0.00000000" sales="7737080.21000000"/>

    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>PRIME MERIDIAN INCOME QP</name>
        <lei>N/A</lei>
        <title>PRIME MERIDIAN INCOME QP</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="PRIMEINC1"/>
        </identifiers>
        <balance>30687.68080000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>3799802.01000000</valUSD>
        <pctVal>3.625608678445</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="PRIVATE"/>
        <issuerConditional desc="private investment" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>Y</isRestrictedSec>

        <fairValLevel>N/A</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>FIRST AMERN FDS INC</name>
        <lei>549300J5J5SJCZ9IA369</lei>
        <title>FRST AM-GV OB-U</title>
        <cusip>31846V211</cusip>
        <identifiers>
          <isin value="US31846V2117"/>
        </identifiers>
        <balance>81972458.23000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>81972458.23000000</valUSD>
        <pctVal>78.21461622737</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Returns are reported without deducting sales loads and redemption fees, if any." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2022-01-28</ncom:dateSigned>
      <ncom:nameOfApplicant>MUTUAL FUND SERIES TRUST</ncom:nameOfApplicant>
      <ncom:signature>Erik Naviloff</ncom:signature>
      <ncom:signerName>Erik Naviloff</ncom:signerName>
      <ncom:title>Treasurer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
