<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd">
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    <isConfidential>false</isConfidential>
    <accessionNumber>0001752724-20-169307</accessionNumber>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001355064</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>
      <seriesClassInfo>
        <seriesId>S000041894</seriesId>
        <classId>C000130114</classId>
        <classId>C000130115</classId>
        <classId>C000130116</classId>
      </seriesClassInfo>

    </filerInfo>
  </headerData>
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      <regName>MUTUAL FUND SERIES TRUST</regName>
      <regFileNumber>811-21872</regFileNumber>
      <regCik>0001355064</regCik>
      <regLei>5493002ZGLQMLR4QMA96</regLei>
      <regStreet1>C/O GEMINI FUND SERVICES LLC</regStreet1>
      <regStreet2>4020 SOUTH 147TH STREET STE 2</regStreet2>
      <regCity>OMAHA</regCity>
      <regStateConditional regCountry="US" regState="US-NE"/>
      <regZipOrPostalCode>68137</regZipOrPostalCode>
      <regPhone>631-549-1859</regPhone>
      <seriesName>Catalyst/Warrington Strategic Program Fund</seriesName>
      <seriesId>S000041894</seriesId>
      <seriesLei>549300HQL3WT31ZF0202</seriesLei>
      <repPdEnd>2020-06-30</repPdEnd>
      <repPdDate>2020-06-30</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
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      <totAssets>145375643.87</totAssets>
      <totLiabs>493732.19</totLiabs>
      <netAssets>144881911.68</netAssets>
      <assetsAttrMiscSec>0.00000000</assetsAttrMiscSec>
      <assetsInvested>0.00000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.00000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.00000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.00000000</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0.00000000</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0.00000000</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0.00000000</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0.00000000</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0.00000000</amtPayAftOneYrOther>
      <delayDeliv>0.00000000</delayDeliv>
      <standByCommit>0.00000000</standByCommit>
      <liquidPref>0.00000000</liquidPref>
      <cshNotRptdInCorD>2837298.39</cshNotRptdInCorD>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000130115" rtn1="-0.28000000" rtn2="0.56000000" rtn3="0.42000000"/>
          <monthlyTotReturn classId="C000130116" rtn1="-0.13000000" rtn2="0.52000000" rtn3="0.52000000"/>
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        </monthlyTotReturns>
        <monthlyReturnCats>
          <equityContracts>
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            <mon3 netRealizedGain="1340112.14000000" netUnrealizedAppr="-92694.50000000"/>
            <futureCategory>
              <instrMon1 netRealizedGain="0.00000000" netUnrealizedAppr="0.00000000"/>
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              <instrMon3 netRealizedGain="0.00000000" netUnrealizedAppr="-70082.00000000"/>
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            <futureCategory>
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              <instrMon3 netRealizedGain="-188215.35000000" netUnrealizedAppr="0.00000000"/>
            </futureCategory>
          </otherContracts>
        </monthlyReturnCats>
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        <othMon2 netRealizedGain="62528.90000000" netUnrealizedAppr="69595.65000000"/>
        <othMon3 netRealizedGain="64405.25000000" netUnrealizedAppr="-63631.82000000"/>
      </returnInfo>
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      <mon2Flow redemption="-5581689.84000000" reinvestment="0.00000000" sales="1668724.61000000"/>
      <mon3Flow redemption="-6969451.99000000" reinvestment="0.00000000" sales="1645544.43000000"/>

    </fundInfo>
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      <invstOrSec>
        <name>OPTION ISSUER</name>
        <lei>N/A</lei>
        <title>S&amp;P 500 EOM OPT   Jul20P</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="EVN0P1750 07/31/2020"/>
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        <balance>-268.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>-77050.00000000</valUSD>
        <pctVal>-0.05318124195</pctVal>
        <payoffProfile>Short</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <optionSwaptionWarrantDeriv derivCat="OPT">
            <counterparties>
              <counterpartyName>HSBC SECURITIES</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <putOrCall>Put</putOrCall>
            <writtenOrPur>Written</writtenOrPur>
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              <nestedDerivInfo>
                <futrDeriv derivCat="FUT">
                  <counterparties>
                    <counterpartyName>N/A</counterpartyName>
                    <counterpartyLei>N/A</counterpartyLei>
                  </counterparties>
                  <derivAddlInfo>
                    <name> Chicago Mercantile Exchange    </name>
                    <lei>N/A</lei>
                    <title>S&amp;P500 EOM OPTION Sep20</title>
                    <cusip>N/A</cusip>
                    <identifiers>
                      <ticker value="EVU0 Ind"/>
                    </identifiers>
                    <balance>250.00000000</balance>
                    <units>NC</units>
                    <curCd>USD</curCd>
                    <valUSD>N/A</valUSD>
                    <pctVal>N/A</pctVal>
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                    <issuerCat>CORP</issuerCat>
                    <invCountry>US</invCountry>
                    <invOthCountry>US</invOthCountry>
                  </derivAddlInfo>
                  <payOffProf>Long</payOffProf>
                  <descRefInstrmnt>
                    <indexBasketInfo>
                      <indexName>CME Standard &amp; Poor's 500 Inde</indexName>
                      <indexIdentifier>SPX</indexIdentifier>
                    </indexBasketInfo>
                  </descRefInstrmnt>
                  <expDate>2020-09-17</expDate>
                  <notionalAmt>193137500.00000000</notionalAmt>
                  <curCd>USD</curCd>
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              </nestedDerivInfo>
            </descRefInstrmnt>
            <shareNo>-268.00000000</shareNo>
            <exercisePrice>1750.00000000</exercisePrice>
            <exercisePriceCurCd>USD</exercisePriceCurCd>
            <expDt>2020-07-31</expDt>
            <delta>XXXX</delta>
            <unrealizedAppr>123950.00000000</unrealizedAppr>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>S&amp;P 500 4th WEEKL Sep20</title>
        <cusip>N/A</cusip>
        <identifiers>
          <ticker value="4DU0"/>
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        <balance>-67.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>-1723575.00000000</valUSD>
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        <payoffProfile>Short</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerConditional desc="future option" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <futrDeriv derivCat="FUT">
            <counterparties>
              <counterpartyName>WEDBUSH SECURITIES</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
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            <payOffProf>Short</payOffProf>
            <descRefInstrmnt>
              <otherRefInst>
                <issuerName>N/A</issuerName>
                <issueTitle>CME S&amp;P 500 European Style Wee</issueTitle>
                <identifiers>
                  <isin value="N/A"/>
                </identifiers>
              </otherRefInst>
            </descRefInstrmnt>
            <expDate>2020-09-17</expDate>
            <notionalAmt>-50037275.00000000</notionalAmt>
            <curCd>USD</curCd>
            <unrealizedAppr>-1723575.00000000</unrealizedAppr>
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        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>United States Treasury Bills</name>
        <lei>254900HROIFWPRGM1V77</lei>
        <title>B 07/30/20</title>
        <cusip>912796WY1</cusip>
        <identifiers>
          <isin value="US912796WY16"/>
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        <balance>25000000.00000000</balance>
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        <curCd>USD</curCd>
        <valUSD>24997583.25000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>UST</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2020-07-30</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>0.00000000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Nuveen Invt Fds Inc</name>
        <lei>N/A</lei>
        <title>FRST AM-GV OB-U</title>
        <cusip>31846V211</cusip>
        <identifiers>
          <isin value="US31846V2117"/>
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        <balance>100759883.32000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>100759883.32000000</valUSD>
        <pctVal>69.54621329303</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>OPTION ISSUER</name>
        <lei>N/A</lei>
        <title>S&amp;P 500 EOM OPT   Jul20P</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="EVN0P2000 07/31/2020"/>
        </identifiers>
        <balance>134.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>61975.00000000</valUSD>
        <pctVal>0.042776216355</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <optionSwaptionWarrantDeriv derivCat="OPT">
            <counterparties>
              <counterpartyName>HSBC SECURITIES</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <putOrCall>Put</putOrCall>
            <writtenOrPur>Purchased</writtenOrPur>
            <descRefInstrmnt>
              <nestedDerivInfo>
                <futrDeriv derivCat="FUT">
                  <counterparties>
                    <counterpartyName>N/A</counterpartyName>
                    <counterpartyLei>N/A</counterpartyLei>
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                  <derivAddlInfo>
                    <name> Chicago Mercantile Exchange    </name>
                    <lei>N/A</lei>
                    <title>S&amp;P500 EOM OPTION Sep20</title>
                    <cusip>N/A</cusip>
                    <identifiers>
                      <ticker value="EVU0 Ind"/>
                    </identifiers>
                    <balance>250.00000000</balance>
                    <units>NC</units>
                    <curCd>USD</curCd>
                    <valUSD>N/A</valUSD>
                    <pctVal>N/A</pctVal>
                    <assetCat>DE</assetCat>
                    <issuerCat>CORP</issuerCat>
                    <invCountry>US</invCountry>
                    <invOthCountry>US</invOthCountry>
                  </derivAddlInfo>
                  <payOffProf>Short</payOffProf>
                  <descRefInstrmnt>
                    <indexBasketInfo>
                      <indexName>CME Standard &amp; Poor's 500 Inde</indexName>
                      <indexIdentifier>SPX</indexIdentifier>
                    </indexBasketInfo>
                  </descRefInstrmnt>
                  <expDate>2020-09-17</expDate>
                  <notionalAmt>193137500.00000000</notionalAmt>
                  <curCd>USD</curCd>
                </futrDeriv>
              </nestedDerivInfo>
            </descRefInstrmnt>
            <shareNo>134.00000000</shareNo>
            <exercisePrice>2000.00000000</exercisePrice>
            <exercisePriceCurCd>USD</exercisePriceCurCd>
            <expDt>2020-07-31</expDt>
            <delta>XXXX</delta>
            <unrealizedAppr>-139025.00000000</unrealizedAppr>
          </optionSwaptionWarrantDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>PRIME MERIDIAN INCOME QP</name>
        <lei>N/A</lei>
        <title>PRIME MERIDIAN INCOME QP</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="PRIMEINC1"/>
        </identifiers>
        <balance>148550.81000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>16202199.17000000</valUSD>
        <pctVal>11.18303795285</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerConditional desc="private investment" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>Y</isRestrictedSec>

        <fairValLevel>N/A</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>S&amp;P EMINI 4th WKL Sep20</title>
        <cusip>N/A</cusip>
        <identifiers>
          <ticker value="4EU0"/>
        </identifiers>
        <balance>335.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>1653493.00000000</valUSD>
        <pctVal>1.141269452360</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerConditional desc="future option" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <derivativeInfo>
          <futrDeriv derivCat="FUT">
            <counterparties>
              <counterpartyName>WEDBUSH SECURITIES</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <payOffProf>Long</payOffProf>
            <descRefInstrmnt>
              <otherRefInst>
                <issuerName>N/A</issuerName>
                <issueTitle>CME E-mini S&amp;P 500 European St</issueTitle>
                <identifiers>
                  <isin value="N/A"/>
                </identifiers>
              </otherRefInst>
            </descRefInstrmnt>
            <expDate>2020-09-18</expDate>
            <notionalAmt>50108194.50000000</notionalAmt>
            <curCd>USD</curCd>
            <unrealizedAppr>1653493.00000000</unrealizedAppr>
          </futrDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Returns are reported without deducting sales loads and redemption fees, if any." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2020-07-28</ncom:dateSigned>
      <ncom:nameOfApplicant>MUTUAL FUND SERIES TRUST</ncom:nameOfApplicant>
      <ncom:signature>Erik Naviloff</ncom:signature>
      <ncom:signerName>Erik Naviloff</ncom:signerName>
      <ncom:title>Treasurer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
