<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd">
  <headerData>
    <submissionType>NPORT-P/A</submissionType>
    <isConfidential>false</isConfidential>
    <accessionNumber>0001752724-21-086938</accessionNumber>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001355064</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>
      <seriesClassInfo>
        <seriesId>S000041894</seriesId>
        <classId>C000130116</classId>
        <classId>C000130115</classId>
        <classId>C000130114</classId>
      </seriesClassInfo>

    </filerInfo>
  </headerData>
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    <genInfo>
      <regName>MUTUAL FUND SERIES TRUST</regName>
      <regFileNumber>811-21872</regFileNumber>
      <regCik>0001355064</regCik>
      <regLei>5493002ZGLQMLR4QMA96</regLei>
      <regStreet1>C/O GEMINI FUND SERVICES LLC</regStreet1>
      <regStreet2>4221 North 203rd Street, Suite 100</regStreet2>
      <regCity>ELKHORN</regCity>
      <regStateConditional regCountry="US" regState="US-NE"/>
      <regZipOrPostalCode>68022</regZipOrPostalCode>
      <regPhone>631-470-2600</regPhone>
      <seriesName>Catalyst/Warrington Strategic Program Fund</seriesName>
      <seriesId>S000041894</seriesId>
      <seriesLei>549300HQL3WT31ZF0202</seriesLei>
      <repPdEnd>2021-06-30</repPdEnd>
      <repPdDate>2020-12-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>108440628.87</totAssets>
      <totLiabs>938937.69</totLiabs>
      <netAssets>107501691.18</netAssets>
      <assetsAttrMiscSec>0.00000000</assetsAttrMiscSec>
      <assetsInvested>0.00000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.00000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.00000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.00000000</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0.00000000</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0.00000000</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0.00000000</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0.00000000</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0.00000000</amtPayAftOneYrOther>
      <delayDeliv>0.00000000</delayDeliv>
      <standByCommit>0.00000000</standByCommit>
      <liquidPref>0.00000000</liquidPref>
      <cshNotRptdInCorD>93871891.97</cshNotRptdInCorD>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000130116" rtn1="0.13000000" rtn2="-0.13000000" rtn3="2.06000000"/>
          <monthlyTotReturn classId="C000130115" rtn1="0.00000000" rtn2="-0.14000000" rtn3="1.94000000"/>
          <monthlyTotReturn classId="C000130114" rtn1="0.13000000" rtn2="-0.13000000" rtn3="1.97000000"/>
        </monthlyTotReturns>
        <monthlyReturnCats>
          <equityContracts>
            <mon1 netRealizedGain="632593.75000000" netUnrealizedAppr="-120350.00000000"/>
            <mon2 netRealizedGain="191325.00000000" netUnrealizedAppr="-7900.00000000"/>
            <mon3 netRealizedGain="1331875.00000000" netUnrealizedAppr="-3100.00000000"/>
            <futureCategory>
              <instrMon1 netRealizedGain="0.00000000" netUnrealizedAppr="0.00000000"/>
              <instrMon2 netRealizedGain="0.00000000" netUnrealizedAppr="0.00000000"/>
              <instrMon3 netRealizedGain="322500.00000000" netUnrealizedAppr="0.00000000"/>
            </futureCategory>
            <optionCategory>
              <instrMon1 netRealizedGain="632593.75000000" netUnrealizedAppr="-120350.00000000"/>
              <instrMon2 netRealizedGain="191325.00000000" netUnrealizedAppr="-7900.00000000"/>
              <instrMon3 netRealizedGain="1009375.00000000" netUnrealizedAppr="-3100.00000000"/>
            </optionCategory>
          </equityContracts>
          <otherContracts>
            <mon1 netRealizedGain="-193388.64000000" netUnrealizedAppr="0.00000000"/>
            <mon2 netRealizedGain="-72493.26000000" netUnrealizedAppr="0.00000000"/>
            <mon3 netRealizedGain="-134838.24000000" netUnrealizedAppr="0.00000000"/>
            <futureCategory>
              <instrMon1 netRealizedGain="-193388.64000000" netUnrealizedAppr="0.00000000"/>
              <instrMon2 netRealizedGain="-72493.26000000" netUnrealizedAppr="0.00000000"/>
              <instrMon3 netRealizedGain="-134838.24000000" netUnrealizedAppr="0.00000000"/>
            </futureCategory>
          </otherContracts>
        </monthlyReturnCats>
        <othMon1 netRealizedGain="99266.27000000" netUnrealizedAppr="-20620.49000000"/>
        <othMon2 netRealizedGain="97579.91000000" netUnrealizedAppr="-149919.77000000"/>
        <othMon3 netRealizedGain="467399.04000000" netUnrealizedAppr="18591.66000000"/>
      </returnInfo>
      <mon1Flow redemption="-5810423.00000000" reinvestment="0.00000000" sales="235014.59000000"/>
      <mon2Flow redemption="-5955313.57000000" reinvestment="0.00000000" sales="837479.64000000"/>
      <mon3Flow redemption="-7695543.68000000" reinvestment="0.00000000" sales="856461.41000000"/>

    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>PRIME MERIDIAN INCOME QP</name>
        <lei>N/A</lei>
        <title>PRIME MERIDIAN INCOME QP</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="PRIMEINC1"/>
        </identifiers>
        <balance>105787.46000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>12331834.74000000</valUSD>
        <pctVal>11.47129371141</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerConditional desc="private investment" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>Y</isRestrictedSec>

        <fairValLevel>N/A</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>OPTION ISSUER</name>
        <lei>N/A</lei>
        <title>S&amp;P 2nd Week Optn Jan21P</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="2DF1P3600 01/08/2021"/>
        </identifiers>
        <balance>320.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>648000.00000000</valUSD>
        <pctVal>0.602781214776</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <optionSwaptionWarrantDeriv derivCat="OPT">
            <counterparties>
              <counterpartyName>ADM INVESTOR SERVICES</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <counterparties>
              <counterpartyName>ROSENTHAL COLLINS GROUP LLC</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <counterparties>
              <counterpartyName>ED&amp;F MAN CAPITAL MARKETS INC</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <putOrCall>Put</putOrCall>
            <writtenOrPur>Purchased</writtenOrPur>
            <descRefInstrmnt>
              <nestedDerivInfo>
                <futrDeriv derivCat="FUT">
                  <counterparties>
                    <counterpartyName>N/A</counterpartyName>
                    <counterpartyLei>N/A</counterpartyLei>
                  </counterparties>
                  <derivAddlInfo>
                    <name> Chicago Mercantile Exchange    </name>
                    <lei>N/A</lei>
                    <title>S&amp;P 500 2ND WEEKL Mar21</title>
                    <cusip>N/A</cusip>
                    <identifiers>
                      <ticker value="2DH1 Ind"/>
                    </identifiers>
                    <balance>250.00000000</balance>
                    <units>NC</units>
                    <curCd>USD</curCd>
                    <valUSD>N/A</valUSD>
                    <pctVal>N/A</pctVal>
                    <assetCat>DE</assetCat>
                    <issuerCat>CORP</issuerCat>
                    <invCountry>US</invCountry>
                    <invOthCountry>US</invOthCountry>
                  </derivAddlInfo>
                  <payOffProf>Short</payOffProf>
                  <descRefInstrmnt>
                    <indexBasketInfo>
                      <indexName>CME Standard &amp; Poor's 500 Inde</indexName>
                      <indexIdentifier>SPX</indexIdentifier>
                    </indexBasketInfo>
                  </descRefInstrmnt>
                  <expDate>2021-03-18</expDate>
                  <notionalAmt>234300000.00000000</notionalAmt>
                  <curCd>USD</curCd>
                </futrDeriv>
              </nestedDerivInfo>
            </descRefInstrmnt>
            <shareNo>320.00000000</shareNo>
            <exercisePrice>3600.00000000</exercisePrice>
            <exercisePriceCurCd>USD</exercisePriceCurCd>
            <expDt>2021-01-08</expDt>
            <delta>XXXX</delta>
            <unrealizedAppr>-344000.00000000</unrealizedAppr>
          </optionSwaptionWarrantDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>FIRST AMERN FDS INC</name>
        <lei>N/A</lei>
        <title>FRST AM-GV OB-U</title>
        <cusip>31846V211</cusip>
        <identifiers>
          <isin value="US31846V2117"/>
        </identifiers>
        <balance>1173903.57000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>1173903.57000000</valUSD>
        <pctVal>1.091986141905</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>OPTION ISSUER</name>
        <lei>N/A</lei>
        <title>S&amp;P 2nd Week Optn Jan21P</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="2DF1P3450 01/08/2021"/>
        </identifiers>
        <balance>-960.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>-660000.00000000</valUSD>
        <pctVal>-0.61394382986</pctVal>
        <payoffProfile>Short</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <optionSwaptionWarrantDeriv derivCat="OPT">
            <counterparties>
              <counterpartyName>ED&amp;F MAN CAPITAL MARKETS INC</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <counterparties>
              <counterpartyName>ROSENTHAL COLLINS GROUP LLC</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <counterparties>
              <counterpartyName>ADM INVESTOR SERVICES</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <putOrCall>Put</putOrCall>
            <writtenOrPur>Written</writtenOrPur>
            <descRefInstrmnt>
              <nestedDerivInfo>
                <futrDeriv derivCat="FUT">
                  <counterparties>
                    <counterpartyName>N/A</counterpartyName>
                    <counterpartyLei>N/A</counterpartyLei>
                  </counterparties>
                  <derivAddlInfo>
                    <name> Chicago Mercantile Exchange    </name>
                    <lei>N/A</lei>
                    <title>S&amp;P 500 2ND WEEKL Mar21</title>
                    <cusip>N/A</cusip>
                    <identifiers>
                      <ticker value="2DH1 Ind"/>
                    </identifiers>
                    <balance>250.00000000</balance>
                    <units>NC</units>
                    <curCd>USD</curCd>
                    <valUSD>N/A</valUSD>
                    <pctVal>N/A</pctVal>
                    <assetCat>DE</assetCat>
                    <issuerCat>CORP</issuerCat>
                    <invCountry>US</invCountry>
                    <invOthCountry>US</invOthCountry>
                  </derivAddlInfo>
                  <payOffProf>Long</payOffProf>
                  <descRefInstrmnt>
                    <indexBasketInfo>
                      <indexName>CME Standard &amp; Poor's 500 Inde</indexName>
                      <indexIdentifier>SPX</indexIdentifier>
                    </indexBasketInfo>
                  </descRefInstrmnt>
                  <expDate>2021-03-18</expDate>
                  <notionalAmt>234300000.00000000</notionalAmt>
                  <curCd>USD</curCd>
                </futrDeriv>
              </nestedDerivInfo>
            </descRefInstrmnt>
            <shareNo>-960.00000000</shareNo>
            <exercisePrice>3450.00000000</exercisePrice>
            <exercisePriceCurCd>USD</exercisePriceCurCd>
            <expDt>2021-01-08</expDt>
            <delta>XXXX</delta>
            <unrealizedAppr>324000.00000000</unrealizedAppr>
          </optionSwaptionWarrantDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>OPTION ISSUER</name>
        <lei>N/A</lei>
        <title>S&amp;P 2nd Week Optn Jan21P</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="2DF1P3250 01/08/2021"/>
        </identifiers>
        <balance>-160.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>-62000.00000000</valUSD>
        <pctVal>-0.05767351129</pctVal>
        <payoffProfile>Short</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <optionSwaptionWarrantDeriv derivCat="OPT">
            <counterparties>
              <counterpartyName>ED&amp;F MAN CAPITAL MARKETS INC</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <counterparties>
              <counterpartyName>ADM INVESTOR SERVICES</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <counterparties>
              <counterpartyName>ROSENTHAL COLLINS GROUP LLC</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
            </counterparties>
            <putOrCall>Put</putOrCall>
            <writtenOrPur>Written</writtenOrPur>
            <descRefInstrmnt>
              <nestedDerivInfo>
                <futrDeriv derivCat="FUT">
                  <counterparties>
                    <counterpartyName>N/A</counterpartyName>
                    <counterpartyLei>N/A</counterpartyLei>
                  </counterparties>
                  <derivAddlInfo>
                    <name> Chicago Mercantile Exchange    </name>
                    <lei>N/A</lei>
                    <title>S&amp;P 500 2ND WEEKL Mar21</title>
                    <cusip>N/A</cusip>
                    <identifiers>
                      <ticker value="2DH1 Ind"/>
                    </identifiers>
                    <balance>250.00000000</balance>
                    <units>NC</units>
                    <curCd>USD</curCd>
                    <valUSD>N/A</valUSD>
                    <pctVal>N/A</pctVal>
                    <assetCat>DE</assetCat>
                    <issuerCat>CORP</issuerCat>
                    <invCountry>US</invCountry>
                    <invOthCountry>US</invOthCountry>
                  </derivAddlInfo>
                  <payOffProf>Long</payOffProf>
                  <descRefInstrmnt>
                    <indexBasketInfo>
                      <indexName>CME Standard &amp; Poor's 500 Inde</indexName>
                      <indexIdentifier>SPX</indexIdentifier>
                    </indexBasketInfo>
                  </descRefInstrmnt>
                  <expDate>2021-03-18</expDate>
                  <notionalAmt>234300000.00000000</notionalAmt>
                  <curCd>USD</curCd>
                </futrDeriv>
              </nestedDerivInfo>
            </descRefInstrmnt>
            <shareNo>-160.00000000</shareNo>
            <exercisePrice>3250.00000000</exercisePrice>
            <exercisePriceCurCd>USD</exercisePriceCurCd>
            <expDt>2021-01-08</expDt>
            <delta>XXXX</delta>
            <unrealizedAppr>9000.00000000</unrealizedAppr>
          </optionSwaptionWarrantDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Returns are reported without deducting sales loads and redemption fees, if any." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2021-01-28</ncom:dateSigned>
      <ncom:nameOfApplicant>MUTUAL FUND SERIES TRUST</ncom:nameOfApplicant>
      <ncom:signature>Erik Naviloff</ncom:signature>
      <ncom:signerName>Erik Naviloff</ncom:signerName>
      <ncom:title>Treasurer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
