<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001355064</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>

      <seriesClassInfo>
        <seriesId>S000036625</seriesId>
        <classId>C000111984</classId>
        <classId>C000142554</classId>
        <classId>C000111983</classId>
      </seriesClassInfo>

    </filerInfo>
  </headerData>
  <formData>
    <genInfo>
      <regName>MUTUAL FUND SERIES TRUST</regName>
      <regFileNumber>811-21872</regFileNumber>
      <regCik>0001355064</regCik>
      <regLei>5493002ZGLQMLR4QMA96</regLei>
      <regStreet1>C/O GEMINI FUND SERVICES LLC</regStreet1>
      <regStreet2>4221 North 203rd Street, Suite 100</regStreet2>
      <regCity>ELKHORN</regCity>
      <regStateConditional regCountry="US" regState="US-NE"/>
      <regZipOrPostalCode>68022</regZipOrPostalCode>
      <regPhone>631-470-2600</regPhone>
      <seriesName>Catalyst/Exceed Defined Risk Fund</seriesName>
      <seriesId>S000036625</seriesId>
      <seriesLei>5493009BMYVTKVDMSN58</seriesLei>
      <repPdEnd>2021-06-30</repPdEnd>
      <repPdDate>2020-09-30</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>10125034.19</totAssets>
      <totLiabs>224189.42</totLiabs>
      <netAssets>9900844.77</netAssets>
      <assetsAttrMiscSec>0.00000000</assetsAttrMiscSec>
      <assetsInvested>0.00000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.00000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.00000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.00000000</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0.00000000</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0.00000000</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0.00000000</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0.00000000</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0.00000000</amtPayAftOneYrOther>
      <delayDeliv>0.00000000</delayDeliv>
      <standByCommit>0.00000000</standByCommit>
      <liquidPref>0.00000000</liquidPref>
      <cshNotRptdInCorD>52754.58000000</cshNotRptdInCorD>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000111983" rtn1="3.46000000" rtn2="2.58000000" rtn3="-2.14000000"/>
          <monthlyTotReturn classId="C000111984" rtn1="3.43000000" rtn2="2.51000000" rtn3="-2.25000000"/>
          <monthlyTotReturn classId="C000142554" rtn1="3.43000000" rtn2="2.65000000" rtn3="-2.12000000"/>
        </monthlyTotReturns>
        <monthlyReturnCats>
          <equityContracts>
            <mon1 netRealizedGain="28902.28000000" netUnrealizedAppr="293802.07000000"/>
            <mon2 netRealizedGain="0.00000000" netUnrealizedAppr="277265.50000000"/>
            <mon3 netRealizedGain="247698.18000000" netUnrealizedAppr="-467824.43000000"/>
            <optionCategory>
              <instrMon1 netRealizedGain="28902.28000000" netUnrealizedAppr="293802.07000000"/>
              <instrMon2 netRealizedGain="0.00000000" netUnrealizedAppr="277265.50000000"/>
              <instrMon3 netRealizedGain="247698.18000000" netUnrealizedAppr="-467824.43000000"/>
            </optionCategory>
          </equityContracts>
        </monthlyReturnCats>
        <othMon1 netRealizedGain="10345.69000000" netUnrealizedAppr="7292.73000000"/>
        <othMon2 netRealizedGain="0.00000000" netUnrealizedAppr="2373.92000000"/>
        <othMon3 netRealizedGain="105272.57000000" netUnrealizedAppr="-128817.82000000"/>
      </returnInfo>
      <mon1Flow redemption="-957462.59000000" reinvestment="0.00000000" sales="984093.87000000"/>
      <mon2Flow redemption="-513482.99000000" reinvestment="0.00000000" sales="747634.35000000"/>
      <mon3Flow redemption="-1589043.78000000" reinvestment="0.00000000" sales="673270.40000000"/>

    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>US BANK MMDA</name>
        <lei>N/A</lei>
        <title>US BANK MMDA-USBGFS 5</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="8AMMF0A92"/>
        </identifiers>
        <balance>4109218.34000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>4109218.34000000</valUSD>
        <pctVal>41.50371443506</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="US Bank Money Market Deposit Account" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Pgim Etf Trust</name>
        <lei>549300OXS91LYTDQF355</lei>
        <title>PGIM ULTRA SHORT</title>
        <cusip>69344A107</cusip>
        <identifiers>
          <isin value="US69344A1079"/>
        </identifiers>
        <balance>17968.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>896962.56000000</valUSD>
        <pctVal>9.059454832761</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Returns are reported without deducting sales loads and redemption fees, if any." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2020-10-28</ncom:dateSigned>
      <ncom:nameOfApplicant>MUTUAL FUND SERIES TRUST</ncom:nameOfApplicant>
      <ncom:signature>Erik Naviloff</ncom:signature>
      <ncom:signerName>Erik Naviloff</ncom:signerName>
      <ncom:title>Treasurer</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
