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        <name>Regeneron Pharmaceuticals Inc</name>
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        <name>Repligen Corp</name>
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      <invstOrSec>
        <name>BioMarin Pharmaceutical Inc</name>
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        <name>Alnylam Pharmaceuticals Inc</name>
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        <name>Natera Inc</name>
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        <name>Neurocrine Biosciences Inc</name>
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        <name>BioNTech SE</name>
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        <name>Halozyme Therapeutics Inc</name>
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        <name>Mettler-Toledo International I</name>
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        <name>Exelixis Inc</name>
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        <name>Waters Corp</name>
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        <name>Corcept Therapeutics Inc</name>
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        <name>United Therapeutics Corp</name>
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        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Illumina Inc</name>
        <lei>SQ95QG8SR5Q56LSNF682</lei>
        <title>Illumina Inc</title>
        <cusip>452327109</cusip>
        <identifiers>
          <isin value="US4523271090"/>
          <ticker value="ILMN"/>
        </identifiers>
        <balance>422578.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>40318166.98000000</valUSD>
        <pctVal>4.007571919994</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Moderna Inc</name>
        <lei>549300EI6OKH5K5Q2G38</lei>
        <title>Moderna Inc</title>
        <cusip>60770K107</cusip>
        <identifiers>
          <isin value="US60770K1079"/>
          <ticker value="MRNA"/>
        </identifiers>
        <balance>1206124.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>33276961.16000000</valUSD>
        <pctVal>3.307685470763</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Alkermes PLC</name>
        <lei>N/A</lei>
        <title>Alkermes PLC</title>
        <cusip>000000000</cusip>
        <identifiers>
          <isin value="IE00B56GVS15"/>
          <ticker value="ALKS"/>
        </identifiers>
        <balance>1170387.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>33484772.07000000</valUSD>
        <pctVal>3.328341597516</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>IE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2025-08-25</ncom:dateSigned>
      <ncom:nameOfApplicant>First Trust Exchange-Traded Fund</ncom:nameOfApplicant>
      <ncom:signature>Derek Maltbie</ncom:signature>
      <ncom:signerName>Derek Maltbie</ncom:signerName>
      <ncom:title>Treasurer, Chief Financial Officer and Chief Accounting Officer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
