SCHEDULE 13G/A 0001104659-23-021843 0001472322 XXXXXXXX LIVE 2 Common Stock, $0.001 par value per share 09/30/2024 0001327273 Lyra Therapeutics, Inc. 55234L105 480 ARSENAL WAY WATERTOWN MA 02472 Rule 13d-1(b) Nantahala Capital Management, LLC MA 0.00 702106.00 0.00 702106.00 702106.00 N 1.0612 IA Wilmot B. Harkey X1 0.00 702106.00 0.00 702106.00 702106.00 N 1.0612 HC Daniel Mack X1 0.00 702106.00 0.00 702106.00 702106.00 N 1.0612 HC Lyra Therapeutics, Inc. 480 ARSENAL WAY WATERTOWN, MA, 02472 (1) Nantahala Capital Management, LLC ("Nantahala") (2) Wilmot B. Harkey (3) Daniel Mack (together the "Reporting Persons") 130 Main St. 2nd Floor, New Canaan, CT 06840 (1) Nantahala is a Massachusetts limited liability company. (2) Each of Messrs. Harkey and Mack is a citizen of the United States of America. N IA HC As of September 30, 2024, Nantahala may be deemed to be the beneficial owner of 702,106 Shares held by funds and separately managed accounts under its control, and as the managing members of Nantahala, each of Messrs. Harkey and Mack may be deemed to be a beneficial owner of those Shares. The 702,106 Shares includes 74,033 Shares which may be acquired by the Reporting Persons within sixty days through the exercise of warrants. As of September 30, 2024, each of the Reporting Persons may be deemed to be the beneficial owner of the following percentage of the total number of Shares outstanding: (1) Nantahala Capital Management, LLC ("Nantahala") : 1.0612% (2) Wilmot B. Harkey: 1.0612% (3) Daniel Mack: 1.0612% (1) Nantahala Capital Management, LLC ("Nantahala") : 0 Shares. (2) Wilmot B. Harkey: 0 Shares. (3) Daniel Mack: 0 Shares. (1) Nantahala Capital Management, LLC ("Nantahala") : 702,106 Shares. (2) Wilmot B. Harkey: 702,106 Shares. (3) Daniel Mack: 702,106 Shares. (1) Nantahala Capital Management, LLC ("Nantahala") : 0 Shares. (2) Wilmot B. Harkey: 0 Shares. (3) Daniel Mack: 0 Shares. (1) Nantahala Capital Management, LLC ("Nantahala") : 702,106 Shares. (2) Wilmot B. Harkey: 702,106 Shares. (3) Daniel Mack: 702,106 Shares. N Y Y N Each of Messrs. Harkey and Mack is filing this Schedule 13G as a control person in respect of shares beneficially owned by Nantahala, an investment adviser as described in ss. 240.13d-1(b)(1)(ii)(E). See Item 4(a). Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Nantahala Capital Management, LLC /s/ Taki Vasilakis 11/14/2024 Wilmot B. Harkey /s/ Wilmot B. Harkey 11/14/2024 Daniel Mack /s/ Daniel Mack 11/14/2024