X0306 4 2022-04-22 0 0001322505 ALBIREO PHARMA, INC. ALBO 0001741553 Duncan Jason C/O ALBIREO PHARMA, INC. 53 STATE STREET, 19TH FLOOR BOSTON MA 02109 0 1 0 0 Chief Legal Officer and GC Common Stock 2022-04-22 4 S 0 240 33.07 D 16423 D Common Stock 2022-04-22 4 S 0 103 32.9257 D 16320 D Common Stock 2022-04-25 4 S 0 221 33.4397 D 16099 D Common Stock 2022-04-25 4 S 0 91 33.4001 D 16008 D Shares sold pursuant to a Rule 10b5-1 trading plan adopted by the reporting person on March 1, 2021. Represents 591 shares of common stock and 15,832 restricted stock units. Shares sold pursuant to a Rule 10b5-1 arrangement established in October 2019 to satisfy tax obligations arising out of the vesting of previously granted restricted stock units. Represents 488 shares of common stock and 15,832 restricted stock units. Represents 579 shares of common stock and 15,520 restricted stock units. Represents 488 shares of common stock and 15,520 restricted stock units. /s/ Jason Duncan 2022-04-25