X0306 4 2022-01-24 0 0001322505 ALBIREO PHARMA, INC. ALBO 0001741553 Duncan Jason C/O ALBIREO PHARMA, INC. 10 POST OFFICE SQUARE, SUITE 1000 BOSTON MA 02109 0 1 0 0 Chief Legal Officer and GC Common Stock 2022-01-24 4 S 0 416 24.8822 D 17935 D Common Stock 2022-01-24 4 S 0 96 25.089 D 17839 D Common Stock 2022-01-24 4 S 0 1176 24.9443 D 16663 D Shares sold pursuant to a Rule 10b5-1 arrangement established in October 2019 to satisfy tax obligations arising out of the vesting of previously granted restricted stock units. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $24.7811 to $24.97, inclusive. The reporting person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, on request, full information regarding the number of shares sold at each separate price within the range set forth above. Represents 1,447 shares of common stock and 16,488 restricted stock units. Represents 1,664 shares of common stock and 16,175 restricted stock units. Shares sold pursuant to a Rule 10b5-1 trading plan adopted by the reporting person on March 1, 2021. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $24.67 to $25.09, inclusive. The reporting person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, on request, full information regarding the number of shares sold at each separate price within the range set forth above. Represents 488 shares of common stock and 16,175 restricted stock units. /s/ Jason Duncan 2022-01-25