UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

SCHEDULE 14A

Proxy Statement Pursuant to Section 14(a) of the

Securities Exchange Act of 1934

Filed by the Registrant  ☒                             Filed by a party other than the Registrant  ☐

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☐	Preliminary Proxy Statement
☐	Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2))
☐	Definitive Proxy Statement
☒	Definitive Additional Materials
☐	Soliciting Material Under §240.14a-12

GLADSTONE INVESTMENT CORPORATION

(Name of Registrant as Specified In Its Charter)

(Name of Person(s) Filing Proxy Statement, If Other Than The Registrant)

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On Friday, July 20, 2018, Gladstone Investment Corporation (the “Company”) mailed a letter to certain of its stockholders of record as of June 4, 2018 (“Record Date Stockholders”) in connection with its 2018 Annual Meeting, urging Record Date Stockholders to cast their votes on the proposals described in the Company’s Definitive Proxy Statement on Schedule 14A (the “Proxy Statement”), which was filed with the Securities and Exchange Commission on June 15, 2018.

The text of the letter is attached below.

July 20, 2018

Dear Fellow Stockholder:

We are writing to remind you that the 2018 Annual Meeting of Stockholders of Gladstone Investment Corporation (the “Company”) is scheduled for Thursday, August 2, 2018 at 11:00 a.m. Eastern Daylight Time (“Eastern Time”). The 2018 Annual Meeting of Stockholders will be a completely “virtual meeting.” You will be able to attend the meeting as well as vote and submit your questions during the live webcast of the meeting by visiting www.virtualshareholdermeeting.com/GAIN2018 and entering the company number and control number included on your proxy card or in the instructions that accompanied your proxy materials.

Our records indicate that as of June 4, 2018, the “Record Date” for the annual meeting, you held shares of the Company and, therefore, you are entitled to vote on the matters as described in the Company’s Definitive Proxy Statement (the “Proxy Statement”) and set forth on the proxy card attached thereto, which were mailed to you and filed with the Securities and Exchange Commission (“SEC”) on June 15, 2018. Our records indicate that we have not yet received your vote.

REMEMBER: Your vote is important, no matter how large or small your holdings may be. Please take a moment to vote your shares.

For your convenience, a duplicate copy of the proxy card or voting instruction form containing your proxy control has been included with this mailing. Stockholders with questions on how to vote are strongly encouraged to contact the Company’s proxy solicitor, Georgeson LLC (“Georgeson”), at (800) 790-6795.

Stockholders also may vote virtually during the live broadcast of the annual meeting on August 2, 2018 at 11:00 a.m. Eastern Time. Please follow the instructions for attending and voting at the annual meeting posted at www.virtualshareholdermeeting.com/GAIN2018. You will need the company number and control number delineated on the enclosed proxy card.

In particular, the Company’s Board of Directors encourages you to vote your shares in favor of all of the proposals described in the Proxy Statement and set forth on the proxy card. Voting promptly will help reduce solicitation costs and will eliminate you receiving follow-up phone calls or mailings.

As the date of the annual meeting approaches, if we have not received your proxy you may receive a call from a representative of Georgeson reminding you to exercise your right to vote.

Thank you in advance for your participation and your consideration in this extremely important matter.

Sincerely,

David Gladstone

Chairman and Chief Executive Officer

Important Information

On June 15, 2018, the Company filed the Proxy Statement with the Securities and Exchange Commission (“SEC”) in connection with the Company’s 2018 Annual Meeting of Stockholders. Stockholders are strongly advised to read the Proxy Statement carefully before making any voting or investment decision because the Proxy Statement contains important information. The Proxy Statement and any other materials filed by the Company with the SEC can be obtained free of charge at the SEC’s website at www.sec.gov or from the Company at www.gladstoneinvestment.com.