<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0303</schemaVersion>

    <documentType>4/A</documentType>

    <periodOfReport>2009-12-08</periodOfReport>

    <dateOfOriginalSubmission>2009-12-15</dateOfOriginalSubmission>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001304161</issuerCik>
        <issuerName>Pharma-Bio Serv, Inc.</issuerName>
        <issuerTradingSymbol>pbsv</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001210052</rptOwnerCik>
            <rptOwnerName>BARRON PARTNERS LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>730 FIFTH AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>25TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10019</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Common Stock Purchase Warrant</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>1.1</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2006-01-25</value>
            </exerciseDate>
            <expirationDate>
                <value>2011-01-25</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock, par value $.0001</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>680800</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>680800</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Common Stock Purchase Warrant</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>1.65</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2006-01-25</value>
            </exerciseDate>
            <expirationDate>
                <value>2011-01-25</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock, par value $.0001</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>680800</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>680800</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <remarks>Form 4 previously filed on December 15, 2009 mistakenly listed in a sale of 30,000 shares of Common Stock on December 11, 2009. Therefore as of the date of the original filing the Reporting Person beneficially owned 2,512,574 shares of Common Stock.</remarks>

    <ownerSignature>
        <signatureName>Andrew B Worden, Managing Director, General Partner Entity</signatureName>
        <signatureDate>2010-01-13</signatureDate>
    </ownerSignature>
</ownershipDocument>
