<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0001301123</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


      <seriesClassInfo>
        <seriesId>S000003278</seriesId>
        <classId>C000008700</classId>
        <classId>C000008699</classId>
      </seriesClassInfo>

    </filerInfo>
  </headerData>
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    <genInfo>
      <regName>Access One Trust</regName>
      <regFileNumber>811-21634</regFileNumber>
      <regCik>0001301123</regCik>
      <regLei>549300B72JMWIA7GNB15</regLei>
      <regStreet1>7501 Wisconsin Avenue Suite 1000</regStreet1>
      <regCity>Bethesda</regCity>
      <regStateConditional regCountry="US" regState="US-MD"/>
      <regZipOrPostalCode>20814</regZipOrPostalCode>
      <regPhone>240-497-6400</regPhone>
      <seriesName>Access Flex Bear High Yield Fund</seriesName>
      <seriesId>S000003278</seriesId>
      <seriesLei>2KPXGP3C38I1ALZ6ZU59</seriesLei>
      <repPdEnd>2020-10-31</repPdEnd>
      <repPdDate>2020-04-30</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
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      <totLiabs>2463.24</totLiabs>
      <netAssets>923461.22</netAssets>
      <assetsAttrMiscSec>0.00000000</assetsAttrMiscSec>
      <assetsInvested>0.00000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.00000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.00000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.00000000</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0.00000000</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0.00000000</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0.00000000</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0.00000000</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0.00000000</amtPayAftOneYrOther>
      <delayDeliv>0.00000000</delayDeliv>
      <standByCommit>0.00000000</standByCommit>
      <liquidPref>0.00000000</liquidPref>
      <cshNotRptdInCorD>386987.37000000</cshNotRptdInCorD>
      <curMetrics>
        <curMetric>
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          <intrstRtRiskdv100 period10Yr="0.00000000" period1Yr="0.00000000" period30Yr="0.00000000" period3Mon="0.00000000" period5Yr="0.00000000"/>
        </curMetric>
      </curMetrics>
      <creditSprdRiskInvstGrade period10Yr="0.00000000" period1Yr="0.00000000" period30Yr="0.00000000" period3Mon="0.00000000" period5Yr="0.00000000"/>
      <creditSprdRiskNonInvstGrade period10Yr="0.00000000" period1Yr="0.00000000" period30Yr="0.00000000" period3Mon="0.00000000" period5Yr="0.00000000"/>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000008699" rtn1="1.00000000" rtn2="8.65000000" rtn3="-2.82000000"/>
          <monthlyTotReturn classId="C000008700" rtn1="0.87000000" rtn2="8.55000000" rtn3="-2.86000000"/>
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        <monthlyReturnCats>
          <creditContracts>
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            <mon2 netRealizedGain="67783.13" netUnrealizedAppr="-1029.04"/>
            <mon3 netRealizedGain="-88079.11" netUnrealizedAppr="-19926.43"/>
            <swapCategory>
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              <instrMon2 netRealizedGain="67783.13" netUnrealizedAppr="-1029.04"/>
              <instrMon3 netRealizedGain="-88079.11" netUnrealizedAppr="-19926.43"/>
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          </creditContracts>
          <interestRtContracts>
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            <mon2 netRealizedGain="-13192.17" netUnrealizedAppr="-11551.55000000"/>
            <mon3 netRealizedGain="-13664.02000000" netUnrealizedAppr="17314.06000000"/>
            <futureCategory>
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              <instrMon2 netRealizedGain="-13192.17" netUnrealizedAppr="-11551.55000000"/>
              <instrMon3 netRealizedGain="-13664.02000000" netUnrealizedAppr="17314.06000000"/>
            </futureCategory>
          </interestRtContracts>
        </monthlyReturnCats>
        <othMon1 netRealizedGain="0.00000000" netUnrealizedAppr="-9.27"/>
        <othMon2 netRealizedGain="0.00000000" netUnrealizedAppr="0.00000000"/>
        <othMon3 netRealizedGain="0.00000000" netUnrealizedAppr="0.00000000"/>
      </returnInfo>
      <mon1Flow redemption="-221905.31000000" reinvestment="0.00000000" sales="411779.46000000"/>
      <mon2Flow redemption="-644961.12000000" reinvestment="0.00000000" sales="1773767.09000000"/>
      <mon3Flow redemption="-1708620.48000000" reinvestment="0.00000000" sales="850211.59000000"/>

    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>UMB FINANCIAL CORP.</name>
        <lei>N/A</lei>
        <title>UMB FINANCIAL CORP.</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="OTHER" value="UMB120501"/>
        </identifiers>
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        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>34000.00000000</valUSD>
        <pctVal>3.681800520004</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>RA</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <repurchaseAgrmt>
          <transCat>Repurchase</transCat>
          <notClearedCentCparty isCleared="N">
            <counterpartyInfos>
              <counterpartyInfo lei="549300MGEH3N3CUCV828" name="UMB FINANCIAL CORP."/>
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          <isTriParty>Y</isTriParty>
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          <maturityDt>2020-05-01</maturityDt>
          <repurchaseCollaterals>
            <repurchaseCollateral>
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              <principalCd>USD</principalCd>
              <collateralVal>34720.66000000</collateralVal>
              <collateralCd>USD</collateralCd>
              <invstCat>UST</invstCat>
            </repurchaseCollateral>
          </repurchaseCollaterals>
        </repurchaseAgrmt>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>CANADIAN IMPERIAL BANK</name>
        <lei>N/A</lei>
        <title>CANADIAN IMPERIAL BANK</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="OTHER" value="CIB120501"/>
        </identifiers>
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        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>52000.00000000</valUSD>
        <pctVal>5.630989030595</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>RA</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <repurchaseAgrmt>
          <transCat>Repurchase</transCat>
          <notClearedCentCparty isCleared="N">
            <counterpartyInfos>
              <counterpartyInfo lei="2IGI19DL77OX0HC3ZE78" name="CANADIAN IMPERIAL BANK"/>
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          </notClearedCentCparty>
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          <repurchaseRt>0.00500000</repurchaseRt>
          <maturityDt>2020-05-01</maturityDt>
          <repurchaseCollaterals>
            <repurchaseCollateral>
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              <invstCat>UST</invstCat>
            </repurchaseCollateral>
          </repurchaseCollaterals>
        </repurchaseAgrmt>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>HSBC SECURITIES (USA), INC.</name>
        <lei>N/A</lei>
        <title>HSBC SECURITIES (USA), INC.</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="OTHER" value="HSB120501"/>
        </identifiers>
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        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>163000.00000000</valUSD>
        <pctVal>17.65098484590</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>RA</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <repurchaseAgrmt>
          <transCat>Repurchase</transCat>
          <notClearedCentCparty isCleared="N">
            <counterpartyInfos>
              <counterpartyInfo lei="CYYGQCGNHMHPSMRL3R97" name="HSBC SECURITIES (USA), INC."/>
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          <repurchaseRt>0.01000000</repurchaseRt>
          <maturityDt>2020-05-01</maturityDt>
          <repurchaseCollaterals>
            <repurchaseCollateral>
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              <invstCat>UST</invstCat>
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          </repurchaseCollaterals>
        </repurchaseAgrmt>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>CDX NORTH AMERICAN HIGH YIELD SERIES 34 V1</name>
        <lei>N/A</lei>
        <title>CDX NORTH AMERICAN HIGH YIELD SERIES 34 V1</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal" value="CDXICES34"/>
        </identifiers>
        <balance>995000.00000000</balance>
        <units>OU</units>
        <descOthUnits>Notional</descOthUnits>
        <curCd>USD</curCd>
        <valUSD>50754.06</valUSD>
        <pctVal>5.496068367657</pctVal>
        <payoffProfile>N/A</payoffProfile>
        <assetCat>DCR</assetCat>
        <issuerConditional desc="IDX CREDIT DEFAULT SWAP" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <swapDeriv derivCat="SWP">
            <counterparties>
              <counterpartyName>CREDIT SUISSE</counterpartyName>
              <counterpartyLei>8TEJM79WW0QKCC0R4P04</counterpartyLei>
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            <descRefInstrmnt>
              <indexBasketInfo>
                <indexName>CDX North America High Yield; Series 34, Version 1, 5 year</indexName>
                <indexIdentifier>CDXICES34</indexIdentifier>
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            </descRefInstrmnt>
            <swapFlag>N</swapFlag>
            <otherRecDesc fixedOrFloating="Other">CDX North America High Yield; Series 34, Version 1, 5 year</otherRecDesc>
            <fixedPmntDesc amount="0.00000000" curCd="USD" fixedOrFloating="Fixed" fixedRt="5.00000000"/>
            <terminationDt>2025-06-20</terminationDt>
            <upfrontPmnt>59543.57000000</upfrontPmnt>
            <pmntCurCd>USD</pmntCurCd>
            <upfrontRcpt>0.00000000</upfrontRcpt>
            <rcptCurCd>USD</rcptCurCd>
            <notionalAmt>995000.00000000</notionalAmt>
            <curCd>USD</curCd>
            <unrealizedAppr>-8789.51</unrealizedAppr>
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        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>United States of America</name>
        <lei>N/A</lei>
        <title>5-YEAR US TREASURY NOTE FUTURE JUN20</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="US912828YE44"/>
          <other otherDesc="Internal" value="FVM0"/>
        </identifiers>
        <balance>-7.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>-690.60000000</valUSD>
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        <payoffProfile>N/A</payoffProfile>
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        <issuerConditional desc="Treasury Future" issuerCat="OTHER"/>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <futrDeriv derivCat="FUT">
            <counterparties>
              <counterpartyName>XCBT</counterpartyName>
              <counterpartyLei>N/A</counterpartyLei>
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            <payOffProf>Short</payOffProf>
            <descRefInstrmnt>
              <otherRefInst>
                <issuerName>United States of America</issuerName>
                <issueTitle>US 5-Year Note (consolidated) Jun 2020</issueTitle>
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                  <ticker value="FVM0"/>
                </identifiers>
              </otherRefInst>
            </descRefInstrmnt>
            <expDate>2020-06-30</expDate>
            <notionalAmt>-877262.53000000</notionalAmt>
            <curCd>USD</curCd>
            <unrealizedAppr>-690.60000000</unrealizedAppr>
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        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>SOCIETE' GENERALE</name>
        <lei>N/A</lei>
        <title>SOCIETE' GENERALE</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="OTHER" value="SOC120501"/>
        </identifiers>
        <balance>237000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>237000.00000000</valUSD>
        <pctVal>25.66431538944</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>RA</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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          <transCat>Repurchase</transCat>
          <notClearedCentCparty isCleared="N">
            <counterpartyInfos>
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          <isTriParty>Y</isTriParty>
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          <maturityDt>2020-05-01</maturityDt>
          <repurchaseCollaterals>
            <repurchaseCollateral>
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              <principalCd>USD</principalCd>
              <collateralVal>241795.35000000</collateralVal>
              <collateralCd>USD</collateralCd>
              <invstCat>UST</invstCat>
            </repurchaseCollateral>
          </repurchaseCollaterals>
        </repurchaseAgrmt>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ROYAL BANK OF CANADA</name>
        <lei>N/A</lei>
        <title>ROYAL BANK OF CANADA</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="OTHER" value="RBC120501"/>
        </identifiers>
        <balance>39000.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>39000.00000000</valUSD>
        <pctVal>4.223241772946</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>RA</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <repurchaseAgrmt>
          <transCat>Repurchase</transCat>
          <notClearedCentCparty isCleared="N">
            <counterpartyInfos>
              <counterpartyInfo lei="ES7IP3U3RHIGC71XBU11" name="ROYAL BANK OF CANADA"/>
            </counterpartyInfos>
          </notClearedCentCparty>
          <isTriParty>Y</isTriParty>
          <repurchaseRt>0.00000000</repurchaseRt>
          <maturityDt>2020-05-01</maturityDt>
          <repurchaseCollaterals>
            <repurchaseCollateral>
              <principalAmt>36700.00000000</principalAmt>
              <principalCd>USD</principalCd>
              <collateralVal>39865.62999999</collateralVal>
              <collateralCd>USD</collateralCd>
              <invstCat>UST</invstCat>
            </repurchaseCollateral>
          </repurchaseCollaterals>
        </repurchaseAgrmt>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <signature>
      <ncom:dateSigned>2020-06-01</ncom:dateSigned>
      <ncom:nameOfApplicant>Access One Trust</ncom:nameOfApplicant>
      <ncom:signature>Christopher E. Sabato</ncom:signature>
      <ncom:signerName>Christopher E. Sabato</ncom:signerName>
      <ncom:title>Treasurer and Principal Financial Officer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
