X0303 4 2011-02-25 0000005768 AMERICAN SCIENCE & ENGINEERING, INC. ASEI 0001293565 Callerame Joseph AMERICAN SCIENCE AND ENGINEERING, INC. 829 MIDDLESEX TURNPIKE BILLERICA MA 01821 0 1 0 0 SVP Common Stock 2011-02-25 4 M 0 2000 39.06 A 16877 D Common Stock 2011-02-25 4 S 0 2000 94.02 D 14877 D Employee Stock Option (Right to Buy) 39.06 2011-02-25 4 M 0 2000 0 D 2014-12-02 Common Stock 2000 8629 D The option vested in three equal installments on December 2, 2005, 2006 and 2007. The price reported is a weighted average price. These shares were sold in multiple transactions at prices ranging from a low of $94.00 to a high of $94.041 for an average of $94.0152. The reporting person undertakes to provide to American Science and Engineering, Inc., any security holder of American Science and Engineering, Inc., or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the ranges set forth in this footnote to this Form 4. M. Colette Cooke, Attorney-in-Fact 2011-02-28