<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2017-03-03</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0001135657</issuerCik>
        <issuerName>Eco-Stim Energy Solutions, Inc.</issuerName>
        <issuerTradingSymbol>ESES</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001290839</rptOwnerCik>
            <rptOwnerName>Chan Lap Wai</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O ALBRIGHT CAPITAL MANAGEMENT, LLC</rptOwnerStreet1>
            <rptOwnerStreet2>601 THIRTEENTH ST, SUITE 1000 SOUTH</rptOwnerStreet2>
            <rptOwnerCity>WASHINGTON</rptOwnerCity>
            <rptOwnerState>DC</rptOwnerState>
            <rptOwnerZipCode>20005</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2017-03-03</value>
            </transactionDate>
            <deemedExecutionDate></deemedExecutionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>J</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
                <transactionShares>
                    <value>2030436</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0.99</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
                    <footnoteId id="F1"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeTransaction>
            <securityTitle>
                <value>Convertible Notes</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>6.00</value>
            </conversionOrExercisePrice>
            <transactionDate>
                <value>2017-03-03</value>
            </transactionDate>
            <deemedExecutionDate></deemedExecutionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>J</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
                <footnoteId id="F2"/>
            </transactionCoding>
            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
                <transactionTotalValue>
                    <value>22000000</value>
                </transactionTotalValue>
                <transactionPricePerShare>
                    <value>22000000</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <exerciseDate>
                <value>2014-10-28</value>
            </exerciseDate>
            <expirationDate>
                <value>2018-05-28</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>3666666</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeTransaction>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">On March 3, 2017, ACM Emerging Markets Master Fund I, L.P. (the &quot;Fund&quot;) sold 2,030,436 shares of common stock of Eco-Stim Energy Solutions, Inc. (the &quot;Issuer&quot;) for an aggregate purchase price of $2,038,045.25 and $22,000,000 aggregate principal amount of convertible secured notes of the Issuer for an aggregate purchase price of $24,354,301.37 in a private transaction with an unaffiliated third party. Albright Capital Management, LLC (the &quot;GP&quot;) is the general partner of the Fund, and may be deemed to beneficially own the securities reported herein to the extent of its direct or indirect interest in the Fund. Pursuant to Rule 16a-1(a)(4) under the Securities Act of 1934, as amended (the &quot;Exchange Act&quot;). This filing shall not be deemed an admission that the GP is, for purposes of Section 16 of the Exchange Act or otherwise, the beneficial owner of any securities in excess of its pecuniary interests, if any.</footnote>
        <footnote id="F2">Due to a 1000 character limit, Footnote 2 is a continuation of Footnote 1: The Reporting Person is a managing director of the GP and accordingly may have an indirect pecuniary interest in the securities of the Issuer beneficially owned by the Fund. Such indirect pecuniary interest cannot be calculated through a proportionate allocation of securities of the Issuer. Therefore, the entire amount of the Fund's interest is being reported. The Reporting Person disclaims beneficial ownership of the securities of the Issuer held by the Fund, except to the extent of his pecuniary interest therein.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Lap Wai Chan</signatureName>
        <signatureDate>2017-03-07</signatureDate>
    </ownerSignature>
</ownershipDocument>
