<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <!-- Generated by Workiva -->
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0002080340</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001288847</issuerCik>
      <issuerName>Five9, Inc.</issuerName>
      <secFileNumber>001-36383</secFileNumber>
      <issuerAddress>
        <com:street1>3001 Bishop Dr. Ste 350</com:street1>
        <com:city>San Ramon</com:city>
        <com:stateOrCountry>CA</com:stateOrCountry>
        <com:zipCode>94583</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>9252012000</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Matthew Tuckness</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Morgan Stanley Smith Barney LLC</name>
        <address>
          <com:street1>Executive Financial Services</com:street1>
          <com:street2>1 New York Plaza 8th Floor</com:street2>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10004</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>5324</noOfUnitsSold>
      <aggregateMarketValue>93010.28</aggregateMarketValue>
      <noOfUnitsOutstanding>76550422</noOfUnitsOutstanding>
      <approxSaleDate>03/04/2026</approxSaleDate>
      <securitiesExchangeName>Nasdaq</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>03/03/2026</acquiredDate>
      <natureOfAcquisitionTransaction>RSU</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>5324</amountOfSecuritiesAcquired>
      <paymentDate>03/03/2026</paymentDate>
      <natureOfPayment>Not Applicable</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Matthew Tuckness</name>
        <address>
          <com:street1>3001 Bishop Dr.</com:street1>
          <com:street2>Suite 350</com:street2>
          <com:city>San Ramon</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94583</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>12/04/2025</saleDate>
      <amountOfSecuritiesSold>5255</amountOfSecuritiesSold>
      <grossProceeds>106939.25</grossProceeds>
    </securitiesSoldInPast3Months>
    <remarks>The sale reported on this Form 144 represents an Issuer mandated sale by the Reporting Person to cover tax withholding obligations in connection with the vesting and settlement of Restricted Stock Units, and it does not represent a discretionary trade by the Reporting Person.</remarks>
    <noticeSignature>
      <noticeDate>03/03/2026</noticeDate>
      <signature>Matthew Tuckness</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
