X0407 4 2023-06-22 0 0001636282 Aeglea BioTherapeutics, Inc. AGLE 0001279281 Magovcevic-Liebisch Ivana C/O AEGLEA BIOTHERAPEUTICS, INC. 805 LAS CIMAS PARKWAY, SUITE 100 AUSTIN TX 78746 1 0 0 0 0 Stock Option (right to buy) 0.30 2023-06-22 5 A 0 E 1950000 0.00 A 2033-06-22 Common Stock 1950000 1950000 D This option award is contingent upon receiving stockholder approval of the amendment and/or restatement of the 2016 Equity Incentive Plan to increase the number of shares available for issuance thereunder. The stock option vests and becomes exercisable in 12 equal monthly installments beginning on July 22, 2023, or on the date of the next annual meeting of stockholders, if earlier, subject to the continuing service of the Reporting Person on each vesting date. /s/ Jonathan Alspaugh, by power of attorney 2023-06-30