Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

Board of Directors

MVB Financial Corp. and Subsidiary

We consent to the incorporation by reference in the registration statements (Nos. 333-189512, 333-186910, 333-145716, and 333-120234) on Forms S-8 and (Nos. 333-180317, 333-208949, and 333-215140) on Forms S-3 of MVB Financial Corp. and Subsidiary of our report, dated March 10, 2017, with respect to the consolidated financial statements of MVB Financial Corp. and Subsidiary and the effectiveness of internal control over financial reporting, which reports appear in MVB Financial Corp.’s 2016 Annual Report on Form 10-K.

 /s/ Dixon Hughes Goodman LLP

Gaithersburg, Maryland

March 10, 2017

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