144 0001276311 XXXXXXXX LIVE 0001625101 PULSE BIOSCIENCES INC 001-37744 601 Brickell Key Drive, Suite 1080 Miami FL 33131 510-906-4600 DARRIN UECKER Director Officer Common Morgan Stanley Smith Barney LLC Executive Financial Services

1 New York Plaza 8th Floor New York NY 10004

120000 1772400.00 67278347 09/11/2025 NASDAQ Common 09/11/2025 Exercise of Stock Options Issuer N 120000 09/11/2025 Cash N DARRIN UECKER

601 Brickell Key Drive, Suite 1080 Miami FL 33131

Common 06/13/2025 15000 239700.00 DARRIN UECKER

601 Brickell Key Drive, Suite 1080 Miami FL 33131

Common 06/12/2025 15000 246000.00 The securities to be sold were and will be received upon the exercise of stock options over the next three months. The dates of acquisition and payment were and will be the dates of exercise/sale. 09/11/2025 06/12/2025 /s/ Darrin Uecker