-----BEGIN PRIVACY-ENHANCED MESSAGE-----
Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
Originator-Key-Asymmetric:
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 TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB
MIC-Info: RSA-MD5,RSA,
 TgPG3xEZeX3Cnn6JvdzyT1YTVHmuFh3ZtfwbOk4zvgnUHfVnbhm5E5wyRCe4IzgF
 0c4ujlIxADIvhs3nVu00cw==

<SEC-DOCUMENT>0001273636-09-000004.txt : 20090716
<SEC-HEADER>0001273636-09-000004.hdr.sgml : 20090716
<ACCEPTANCE-DATETIME>20090716125226
ACCESSION NUMBER:		0001273636-09-000004
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
FILED AS OF DATE:		20090716
DATE AS OF CHANGE:		20090716
EFFECTIVENESS DATE:		20090716

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			APPLIED GENETIC TECHNOLOGIES CORP
		CENTRAL INDEX KEY:			0001273636
		IRS NUMBER:				593553710
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			0630

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-61188
		FILM NUMBER:		09947660

	BUSINESS ADDRESS:	
		STREET 1:		11801 RESEARCH DRIVE
		STREET 2:		SUITE D
		CITY:			ALACHUA
		STATE:			FL
		ZIP:			32615
		BUSINESS PHONE:		386-462-2204

	MAIL ADDRESS:	
		STREET 1:		11801 RESEARCH DRIVE
		STREET 2:		SUITE D
		CITY:			ALACHUA
		STATE:			FL
		ZIP:			32615
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0401</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001273636</cik>
        <entityName>APPLIED GENETIC TECHNOLOGIES CORP</entityName>
        <issuerAddress>
            <street1>11801 RESEARCH DRIVE</street1>
            <street2>SUITE D</street2>
            <city>ALACHUA</city>
            <stateOrCountry>FL</stateOrCountry>
            <zipCode>32615</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>386-462-2204</issuerPhoneNumber>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>SUSAN</firstName>
                <middleName>B</middleName>
                <lastName>WASHER</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>11801 Research Drive</street1>
                <street2>SUITE D</street2>
                <city>ALACHUA</city>
                <stateOrCountry>FL</stateOrCountry>
                <zipCode>32615</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>SCOTT</firstName>
                <middleName></middleName>
                <lastName>KOENIG</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>11801 RESEARCH DRIVE</street1>
                <street2>SUITE D</street2>
                <city>ALACHUA</city>
                <stateOrCountry>FL</stateOrCountry>
                <zipCode>32615</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>GARHENG</firstName>
                <middleName></middleName>
                <lastName>KONG</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>406 BLACKWELL STREET</street1>
                <street2>SUITE 200</street2>
                <city>DURHAM</city>
                <stateOrCountry>NC</stateOrCountry>
                <zipCode>27701</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>FRANK</firstName>
                <middleName></middleName>
                <lastName>TOP</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>ONE MEDIMMUNE WAY</street1>
                <street2></street2>
                <city>GAITHERSBURG</city>
                <stateOrCountry>MD</stateOrCountry>
                <zipCode>20878</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ARNOLD</firstName>
                <middleName></middleName>
                <lastName>ORONSKY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2710 SAND HILL ROAD</street1>
                <street2>SECOND FLOOR</street2>
                <city>MENLO PARK</city>
                <stateOrCountry>CA</stateOrCountry>
                <zipCode>94025</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>NICHOLAS</firstName>
                <middleName></middleName>
                <lastName>MUZYCZKA</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>11801 RESEARCH DRIVE</street1>
                <street2>SUITE D</street2>
                <city>ALACHUA</city>
                <stateOrCountry>FL</stateOrCountry>
                <zipCode>32615</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Biotechnology</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>9999999997-07-031617</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2003-11-25</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>38799998</totalOfferingAmount>
            <totalAmountSold>33549998</totalAmountSold>
            <totalRemaining>5250000</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>11</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>1250000</dollarAmount>
                <isEstimate>true</isEstimate>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>APPLIED GENETIC TECHNOLOGIES CORP</issuerName>
                <signatureName>SUSAN B WASHER</signatureName>
                <nameOfSigner>SUSAN B WASHER</nameOfSigner>
                <signatureTitle>PRESIDENT AND CHIEF EXECUTIVE OFFICER</signatureTitle>
                <signatureDate>2009-07-16</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----