<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2022-08-21</periodOfReport>

    <issuer>
        <issuerCik>0001273636</issuerCik>
        <issuerName>APPLIED GENETIC TECHNOLOGIES CORP</issuerName>
        <issuerTradingSymbol>AGTC</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001867439</rptOwnerCik>
            <rptOwnerName>Reynolds Gerald Anthony</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O APPLIED GENETIC TECHNOLOGIES CORP.</rptOwnerStreet1>
            <rptOwnerStreet2>14193 NW 119TH TERRACE, SUITE #10</rptOwnerStreet2>
            <rptOwnerCity>ALACHUA</rptOwnerCity>
            <rptOwnerState>FL</rptOwnerState>
            <rptOwnerZipCode>32615</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Chief Accounting Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2022-08-21</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>30000</value>
                    <footnoteId id="F1"/>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>51006</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Represents a grant of restricted stock units (&quot;RSUs&quot;) under the Applied Genetic Technologies Corporation (&quot;AGTC&quot;) 2013 Equity and Incentive Plan. Each RSU represents the contingent right to receive, upon vesting of the unit, one share of AGTC common stock. The RSUs vest in two installments, with 50% vesting on August 21, 2023 and the remaining 50% vesting on August 21, 2024, subject to the Reporting Person's continued employment with or performance of services for the Company as of each vesting date.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Stacie Aarestad, Attorney-in-fact</signatureName>
        <signatureDate>2022-08-24</signatureDate>
    </ownerSignature>
</ownershipDocument>
