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Proc-Type: 2001,MIC-CLEAR
Originator-Name: webmaster@www.sec.gov
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MIC-Info: RSA-MD5,RSA,
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<SEC-DOCUMENT>0000898432-10-000208.txt : 20100224
<SEC-HEADER>0000898432-10-000208.hdr.sgml : 20100224
<ACCEPTANCE-DATETIME>20100224102043
ACCESSION NUMBER:		0000898432-10-000208
CONFORMED SUBMISSION TYPE:	D/A
PUBLIC DOCUMENT COUNT:		1
ITEM INFORMATION:		Rule 506
ITEM INFORMATION:		Investment Company Act Section 3(c)
ITEM INFORMATION:		Section 3(c)(7)
FILED AS OF DATE:		20100224
DATE AS OF CHANGE:		20100224
EFFECTIVENESS DATE:		20100224

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			OSPREY HIGH YIELD OPPORTUNITY FUND LP
		CENTRAL INDEX KEY:			0001256004
		IRS NUMBER:				000000000
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	FILING VALUES:
		FORM TYPE:		D/A
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	021-57154
		FILM NUMBER:		10628515

	BUSINESS ADDRESS:	
		STREET 1:		801 12TH AVENUE S
		STREET 2:		SUITE 200
		CITY:			NAPLES
		STATE:			FL
		ZIP:			34102
		BUSINESS PHONE:		239-263-6877

	MAIL ADDRESS:	
		STREET 1:		600 5TH AVENUE SOUTH
		STREET 2:		SUITE 210
		CITY:			NAPLES
		STATE:			FL
		ZIP:			34102
</SEC-HEADER>
<DOCUMENT>
<TYPE>D/A
<SEQUENCE>1
<FILENAME>primary_doc.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0603</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001256004</cik>
        <entityName>OSPREY HIGH YIELD OPPORTUNITY FUND LP</entityName>
        <issuerAddress>
            <street1>600 5TH AVENUE SOUTH</street1>
            <street2>SUITE 210</street2>
            <city>NAPLES</city>
            <stateOrCountry>FL</stateOrCountry>
            <zipCode>34102</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>239-263-6877</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Partnership</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>N/A</firstName>
                <lastName>Osprey Asset Management, LLC</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 5th Avenue South</street1>
                <street2>Suite 210</street2>
                <city>Naples</city>
                <stateOrCountry>FL</stateOrCountry>
                <zipCode>34102</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>General Partner</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>N/A</firstName>
                <lastName>Wasmer Schroeder &amp; Company, Inc.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 5th Avenue</street1>
                <street2>Suite 210</street2>
                <city>Naples</city>
                <stateOrCountry>FL</stateOrCountry>
                <zipCode>34102</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Member of the General Partner</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Martin</firstName>
                <middleName>M.</middleName>
                <lastName>Wasmer</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 5th Avenue</street1>
                <street2>Suite 210</street2>
                <city>Naples</city>
                <stateOrCountry>FL</stateOrCountry>
                <zipCode>34102</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Manager of General Partner; Officer and Director of Member</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Michael</firstName>
                <middleName>J.</middleName>
                <lastName>Schroeder</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 5th Avenue</street1>
                <street2>Suite 210</street2>
                <city>Naples</city>
                <stateOrCountry>FL</stateOrCountry>
                <zipCode>34102</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Manager of General Partner; Officer and Director of Member</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pooled Investment Fund</industryGroupType>
            <investmentFundInfo>
                <investmentFundType>Hedge Fund</investmentFundType>
                <is40Act>false</is40Act>
            </investmentFundInfo>
        </industryGroup>
        <issuerSize>
            <aggregateNetAssetValueRange>Decline to Disclose</aggregateNetAssetValueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
            <item>3C</item>
            <item>3C.7</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>9999999997-03-030158</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2003-06-30</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isPooledInvestmentFundType>true</isPooledInvestmentFundType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>250000</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>Indefinite</totalOfferingAmount>
            <totalAmountSold>67981500</totalAmountSold>
            <totalRemaining>Indefinite</totalRemaining>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>92</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
                <isEstimate>true</isEstimate>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
                <isEstimate>true</isEstimate>
            </findersFees>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>OSPREY HIGH YIELD OPPORTUNITY FUND LP</issuerName>
                <signatureName>/s/ Michael J. Schroeder</signatureName>
                <nameOfSigner>Michael J. Schroeder</nameOfSigner>
                <signatureTitle>Manager of the General Partner</signatureTitle>
                <signatureDate>2010-02-10</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
-----END PRIVACY-ENHANCED MESSAGE-----