Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

The Board of Directors

GlobeImmune, Inc.:

We consent to the incorporation by reference in the registration statement (No. 333-202820) on Form S-8 of GlobeImmune, Inc. of our report dated March 15, 2016, with respect to the balance sheet of GlobeImmune, Inc. as of December 31, 2015, and the related statements of operations, stockholders’ equity, and cash flows for the year then ended, which report appears in the December 31, 2015 annual report on Form 10-K of GlobeImmune, Inc.

/s/ EKS&H LLLP

Boulder, Colorado

March 15, 2016