X0306 4 2021-10-18 0 0001563880 Trevi Therapeutics, Inc. TRVI 0001237289 SANDELL SCOTT D 1954 GREENSPRING DRIVE SUITE 600 TIMONIUM MD 21093 0 0 1 0 Common Stock 2021-10-18 4 P 0 1851852 A 7789850 I See Note 2 3.5-Year Common Stock Warrant 1.37 2021-10-18 4 P 0 1851852 A 2021-10-18 2025-04-18 Common Stock 1851852 1851852 I See Note 2 7-Year Common Stock Warrant 1.37 2021-10-18 4 P 0 1851852 A 2021-10-18 2028-10-18 Common Stock 1851852 1851852 I See Note 2 Represents securities acquired pursuant to a Securities Purchase Agreement (the "SPA"), dated October 15, 2021, by and between the Issuer and New Enterprise Associates 16, L.P. ("NEA 16"), with a closing on October 18, 2021. Pursuant to the terms of the SPA, NEA 16 purchased common units for $1.62 per common unit. Each common unit consisted of one share of the Issuer's common stock, one 3.5 year common stock warrant, and one 7-year common stock warrant. The Reporting Person is a manager of NEA 16 GP, LLC, which is the sole general partner of NEA Partners 16, L.P. ("NEA Partners 16"). NEA Partners 16 is the sole general partner of NEA 16, the direct beneficial owner of the securities. The Reporting Person disclaims beneficial ownership within the meaning of Section 16 of the Securities Exchange Act of 1934, as amended, or otherwise of such portion of the securities held by NEA 16 in which the Reporting Person has no pecuniary interest. /s/ Sasha Keough, attorney-in-fact 2021-10-19