SCHEDULE 13D/A 0001224962 XXXXXXXX LIVE 8 Common Stock, $0.001 par value 11/11/2025 false 0001327273 55234L204 Lyra Therapeutics, Inc.

480 Arsenal Way Watertown MA 02472

Alexander Rakitin (646) 205-5345 51 Astor Place, 10th Floor New York NY 10003 0001224962 N Perceptive Advisors LLC b AF N DE 0 138885 0 138885 138885 N 7.8 IA 0001164426 N Joseph Edelman b AF N X1 0 138885 0 138885 138885 N 7.8 IN 0001249675 N Perceptive Life Sciences Master Fund, Ltd. b WC N E9 0 124859 0 124859 124859 N 7.0 CO 0001797811 N Perceptive LS (A), LLC b WC N E9 0 14026 0 14026 14026 N 0.8 CO Common Stock, $0.001 par value Lyra Therapeutics, Inc. 480 Arsenal Way Watertown MA 02472 Items 5(a)-(c) of the Schedule 13D are amended and supplemented as follows: The information set forth in rows 11 and 13 of the cover pages to this Schedule 13D is incorporated by reference. The percentage set forth in row 13 is based on 1,774,882 outstanding shares of Common Stock as of November 7, 2025 as reported by the Issuer in its Quarterly Report on Form 10-Q filed with the Securities and Exchange Commission on November 12, 2025. The information set forth in rows 7 through 10 of the cover pages to this Schedule 13D is incorporated by reference. Except as set forth on Schedule A, no Reporting Person has effected any transaction in shares of Common Stock since the filing of Amendment No. 7 to the Schedule 13D, filed with the Securities and Exchange Commission on November 10, 2025. Perceptive Advisors LLC /s/ Joseph Edelman 11/13/2025 Joseph Edelman /s/ Joseph Edelman 11/13/2025 Perceptive Life Sciences Master Fund, Ltd. /s/ Joseph Edelman 11/13/2025 Perceptive LS (A), LLC Perceptive LS GP, LLC 11/13/2025 /s/ Joseph Edelman 11/13/2025