X0609 4/A 2026-05-11 2026-05-11 0001625101 PULSE BIOSCIENCES, INC. PLSE 0001191249 LAVIOLETTE PAUL A false C/O PULSE BIOSCIENCES, INC. 601 BRICKELL KEY DRIVE, SUITE 1080 MIAMI FL 33131 1 1 Chief Executive Officer 0 Common Stock 2026-05-11 4 P 0 15000 19.69 A 15846 D The shares reported herein were acquired through the Company's at-the-market equity offering program established pursuant to that certain Equity Distribution Agreement, dated February 19, 2026, by and between the Company and TD Securities (USA) LLC, as sales agent. The shares were purchased during an open trading window under the Company's Insider Trading Policy and in accordance with applicable securities laws. The shares reported herein were acquired in a negotiated through the Company's at-the-market equity offering program at the prevailing market bid price or better at the time of sale. Includes shares purchased through the Company's Employee Stock Purchase Plan in March 2026. /s/ Kenneth B. Stratton, as Attorney-in-Fact 2026-05-11