The Securities and Exchange Commission has not necessarily reviewed the information in this filing and has not determined if it is accurate and complete.
The reader should not assume that the information is accurate and complete.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM 13F

FORM 13F COVER PAGE

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OMB Number: 3235-0006
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Report for the Calendar Year or Quarter Ended: 12-31-2023
Check here if Amendment    Amendment Number:
This Amendment (Check only one.):    is a restatement.
   adds new holdings entries.
Institutional Investment Manager Filing this Report:
Name: TWO SIGMA INVESTMENTS, LP
Address: 100 Avenue of the Americas
16th Floor
New York, NY  10013
Form 13F File Number: 028-10385
CRD Number (if applicable): 000137137
SEC File Number (if applicable): 801-70476

The institutional investment manager filing this report and the person by whom it is signed hereby represent that the person signing the report is authorized to submit it, that all information contained herein is true, correct and complete, and that it is understood that all required items, statements, schedules, lists, and tables, are considered integral parts of this form.

Person Signing this Report on Behalf of Reporting Manager:
Name: Steve H. Metzger
Title: Managing Director, Co-General Counsel
Phone: 212-625-5700
Signature, Place, and Date of Signing:
/s/ Steve H. Metzger New YorkNY 02-14-2024
[Signature] [City, State] [Date]
This Form 13F holdings report is being filed on behalf of Two Sigma Investments, LP ("Investments"). Investments serves as the sole manager or investment manager, as applicable, of (i) Two Sigma Equity Portfolio, LLC ("Equity"), Two Sigma Spectrum Portfolio, LLC ("Spectrum"), Two Sigma Futures Portfolio, LLC ("Futures"), Two Sigma Horizon Portfolio, LLC ("Horizon"), Two Sigma Blazar Portfolio, LLC ("Blazar"), Two Sigma Navigator Portfolio, LLC ("Navigator") and Two Sigma Equity Spectrum Portfolio, LLC ("Equity Spectrum"), seven investment entities that are managed by Investments, each of which may be deemed to be an "other manager" for purposes of this Form 13F report and (ii) Two Sigma Galileo Portfolio, LLC ("Galileo"), Two Sigma Pioneer Portfolio, LLC ("Pioneer") and Two Sigma Discovery Portfolio, LLC ("Discovery") three investment entities that are managed by Investments and that each may not presently be deemed to be an "other manager" for purposes of this Form 13F report. Investments, rather than Equity, Spectrum, Futures, Horizon, Blazar, Navigator, Equity Spectrum, Galileo, Pioneer or Discovery exercises sole investment discretion and voting authority over the securities held in the various accounts of Equity, Spectrum, Futures, Horizon, Blazar, Navigator, Equity Spectrum, Galileo, Pioneer and Discovery.
Report Type (Check only one.):
X 13F HOLDINGS REPORT. (Check here if all holdings of this reporting manager are reported in this report.)
   13F NOTICE. (Check here if no holdings reported are in this report, and all holdings are reported by other reporting manager(s).)
   13F COMBINATION REPORT. (Check here if a portion of the holdings for this reporting manager are reported in this report and a portion are reported by other reporting manager(s).)
Form 13F Summary Page
Report Summary:
Number of Other Included Managers: 7
Form 13F Information Table Entry Total: 3,116
Form 13F Information Table Value Total: 43,537,680,602
(round to nearest dollar)
List of Other Included Managers:
Provide a numbered list of the name(s) and Form 13F file number(s) of all institutional investment managers with respect to which this report is filed, other than the manager filing this report.
[If there are no entries in this list, state “NONE” and omit the column headings and list entries.]
No. Name Form 13F File No. CRD No. (if applicable) SEC File No. (if applicable) CIK
1 TWO SIGMA EQUITY PORTFOLIO LLC 028-10704     0001280134
2 TWO SIGMA SPECTRUM PORTFOLIO, LLC 028-11144     0001316047
3 Two Sigma Futures Portfolio, LLC 028-16011     0001600096
4 TWO SIGMA HORIZON PORTFOLIO, LLC 028-15356     0001453226
5 Two Sigma Equity Spectrum Portfolio, LLC 028-20138     0001802995
6 Two Sigma Blazar Portfolio, LLC 028-23019     0001965609
7 Two Sigma Navigator Portfolio, LLC 028-23021     0001965593